一种准确测定与猪免疫球蛋白反应的单克隆抗体特异性的方法。

A method for the accurate determination of the specificity of monoclonal antibodies reactive with porcine immunoglobulins.

作者信息

Hollingshead M G, Tonkonogy S L, De Buysscher E V

出版信息

Vet Immunol Immunopathol. 1985 Nov;10(2-3):167-75. doi: 10.1016/0165-2427(85)90044-3.

Abstract

Monoclonal antibodies against porcine IgG were produced by fusion and characterized. The supernatants of microtiter wells containing fusion hybrids were first screened with an ELISA using semi-purified porcine IgG as antigen. Hybrids reactive in ELISA were cloned by limiting dilution. Further characterization of the specificity of the monoclonal antibodies was done by a combination of two methods: SDS-PAGE electroimmunoblotting and convection blotting of immunoelectrophoretic patterns (IEP-immunoblotting). Using these techniques, we identified monoclonal antibodies specific for porcine Ig gamma chains.

摘要

通过融合制备了抗猪IgG的单克隆抗体并进行了表征。首先，使用半纯化的猪IgG作为抗原，通过ELISA对含有融合杂交体的微量滴定板上清液进行筛选。通过有限稀释法对ELISA中有反应的杂交体进行克隆。单克隆抗体特异性的进一步表征通过两种方法结合进行：SDS-PAGE免疫印迹和免疫电泳图谱的对流印迹（IEP免疫印迹）。使用这些技术，我们鉴定出了对猪Igγ链具有特异性的单克隆抗体。