Hohmann A, Peters V, Comacchio R, Bradley J
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, Australia.
Mol Immunol. 1993 Apr;30(6):521-7. doi: 10.1016/0161-5890(93)90026-8.
Two mouse monoclonal antibodies (VIC5 and VIC6; referred to as Ab1) reacting with the p24 core antigen of HIV-1 were used to produce mouse monoclonal anti-idiotypic antibodies (Ab2). Six anti-idiotypic antibodies were characterized. The five anti-idiotypic antibodies directed against VIC6 partly competed which each other and thus defined a set of overlapping idiotypes on Ab1. All 6 Ab2s inhibited the binding of the corresponding anti-p24 antibody to antigen, although four (W1, Y16, Y6, X14) were markedly more inhibitory than the remaining two (G6, Y11). All six Ab2s were antigen-inhibitable; however the interaction of G6 and Y11 with Ab1 was blocked with considerably less soluble p24 antigen than the remaining four. Correspondingly, G6 and Y11 had lower affinities for Ab1 than did W1, Y6 and X14; the affinity index of Y16 was equivalent to that of Y11. None of the Ab2s reacted with H or L chains of Ab1 after reduction on SDS-gels. Similarly, both Ab1s failed to react with the H or L chains of Ab2. These criteria appeared to define at least four of these Ab2s as internal image antibodies whose image is composed of both H and L chains. The anti-idiotypic antibodies were injected either individually or as a combined preparation of all 6 into syngeneic mice and Porton rats. Despite the presence of anti-anti-idiotypic antibodies (Ab3) in these animals, when used individually no antigen-specific antibodies were found. A small response to p24 antigen was induced in 3 of 6 mice using preparations containing all 6 anti-idiotypes.
使用两种与HIV-1的p24核心抗原发生反应的小鼠单克隆抗体（VIC5和VIC6；称为Ab1）来制备小鼠单克隆抗独特型抗体（Ab2）。对六种抗独特型抗体进行了特性鉴定。针对VIC6的五种抗独特型抗体彼此部分竞争，从而在Ab1上定义了一组重叠的独特型。所有6种Ab2均抑制相应的抗p24抗体与抗原的结合，尽管四种（W1、Y16、Y6、X14）的抑制作用明显强于其余两种（G6、Y11）。所有六种Ab2均可被抗原抑制；然而，与其余四种相比，G6和Y11与Ab1的相互作用被可溶性p24抗原阻断的程度要低得多。相应地，G6和Y11对Ab1的亲和力低于W1、Y6和X14；Y16的亲和指数与Y11相当。在SDS凝胶上还原后，没有一种Ab2与Ab1的重链或轻链发生反应。同样，两种Ab1也未能与Ab2的重链或轻链发生反应。这些标准似乎将其中至少四种Ab2定义为内影像抗体，其影像由重链和轻链组成。将抗独特型抗体单独或作为所有6种的联合制剂注射到同基因小鼠和波顿大鼠体内。尽管这些动物体内存在抗抗独特型抗体（Ab3），但单独使用时未发现抗原特异性抗体。使用包含所有6种抗独特型的制剂，在6只小鼠中的3只中诱导出了对p24抗原的微弱反应。
