Rejeki Purwo Sri, Pranoto Adi, Widiatmaja Deandra Maharani, Utami Dita Mega, Izzatunnisa Nabilah, Lesmana Ronny, Halim Shariff
Physiology Division, Department of Medical Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, East Java, Indonesia.
Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, East Java, Indonesia.
Sports (Basel). 2024 Apr 25;12(5):116. doi: 10.3390/sports12050116.
The integration of combined aerobic exercise and intermittent fasting (IF) has emerged as a strategy for the prevention and management of obesity, including its associated health issues such as age-related metabolic diseases. This study aimed to examine the potential of combined aerobic exercise and IF as a preventative strategy against cellular senescence by targeting mTOR and Bcl-2 levels in obese females. A total of 30 obese women, aged 23.56 ± 1.83 years, body fat percentage (FAT) 45.21 ± 3.73% (very high category), BMI 30.09 ± 3.74 kg/m were recruited and participated in three different types of interventions: intermittent fasting (IF), exercise (EXG), and a combination of intermittent fasting and exercise (IFEXG). The intervention program was carried out 5x/week for 2 weeks. We examined mTOR and Bcl-2 levels using ELISA kits. Statistical analysis used the one-way ANOVA test and continued with Tukey's HSD post hoc test, with a significance level of 5%. The study results showed that a combination of aerobic exercise and IF significantly decreased mTOR levels (-1.26 ± 0.79 ng/mL) compared to the control group (-0.08 ± 1.33 ng/mL; ≤ 0.05). However, combined aerobic exercise and IF did not affect Bcl-2 levels significantly (-0.07 ± 0.09 ng/mL) compared to the control group (0.01 ± 0.17 ng/mL, ≥ 0.05). The IF-only group, exercise-only group, and combined group all showed a significant decrease in body weight and fat mass compared to the control group ( ≤ 0.05). However, the combined aerobic exercise and IF program had a significant effect in reducing the total percentage of body fat and fat mass compared to the IF-only group ( ≤ 0.05). Therefore, it was concluded that the combined intermittent fasting and exercise group (IFEXG) undertook the most effective intervention of the three in terms of preventing cellular senescence, as demonstrated by decreases in the mTOR level, body weight, and fat mass. However, the IFEXG did not present reduced Bcl-2 levels.
有氧运动与间歇性禁食（IF）相结合已成为一种预防和管理肥胖及其相关健康问题（如与年龄相关的代谢性疾病）的策略。本研究旨在通过针对肥胖女性的mTOR和Bcl-2水平，探讨有氧运动与间歇性禁食相结合作为预防细胞衰老策略的潜力。共招募了30名肥胖女性，年龄23.56±1.83岁，体脂百分比（FAT）为45.21±3.73%（极高类别），BMI为30.09±3.74kg/m，并参与了三种不同类型的干预：间歇性禁食（IF）、运动（EXG）以及间歇性禁食与运动相结合（IFEXG）。干预计划每周进行5次，共持续2周。我们使用ELISA试剂盒检测mTOR和Bcl-2水平。统计分析采用单因素方差分析，并继续进行Tukey's HSD事后检验，显著性水平为5%。研究结果表明，与对照组（-0.08±1.33 ng/mL；P≤0.05）相比，有氧运动与间歇性禁食相结合显著降低了mTOR水平（-1.26±0.79 ng/mL）。然而，与对照组（0.01±0.17 ng/mL，P≥0.05）相比，有氧运动与间歇性禁食相结合对Bcl-2水平没有显著影响（-0.07±0.09 ng/mL）。与对照组相比，仅进行间歇性禁食组、仅进行运动组和联合组的体重和脂肪量均显著下降（P≤0.05）。然而，与仅进行间歇性禁食组相比，有氧运动与间歇性禁食相结合的方案在降低体脂和脂肪量的总百分比方面具有显著效果（P≤0.05）。因此，得出的结论是，在预防细胞衰老方面，间歇性禁食与运动相结合组（IFEXG）是三种干预措施中最有效的，这体现在mTOR水平、体重和脂肪量的降低上。然而，IFEXG组的Bcl-2水平并未降低。
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