人纤维肉瘤细胞系MR-83的建立与部分特性鉴定

Establishment and partial characterisation of a human fibrosarcoma cell line MR-83.

作者信息

Stuckly K, Kiddle R, Leversha M, Ellis W M, Ekert H

出版信息

Aust J Exp Biol Med Sci. 1985 Aug;63 ( Pt 4):431-8. doi: 10.1038/icb.1985.48.

DOI:10.1038/icb.1985.48

Abstract

We describe a mycoplasma-free human fibrosarcoma cell line, MR-83, which grows readily in liquid culture and as clones in semi-solid agar with a plating efficiency of about 0.5%. It has a stable karyotype consisting of a modal number of 49-51 chromosomes, with two translocations and a deletion. The cell line shows resistance to adriamycin in semi-solid agar assay, and responds to Epidermal Growth Factor (EGF) by increased DNA synthesis, as measured by thymidine uptake.

摘要

我们描述了一种无支原体的人纤维肉瘤细胞系MR - 83，它在液体培养中易于生长，并能在半固体琼脂中形成克隆，平板接种效率约为0.5%。其核型稳定，众数染色体数目为49 - 51条，有两个易位和一个缺失。在半固体琼脂试验中，该细胞系对阿霉素有抗性，并且如通过胸苷摄取所测定的，对表皮生长因子（EGF）有反应，表现为DNA合成增加。