Melick R A, Farrugia W, Quelch K J
Aust N Z J Med. 1985 Aug;15(4):410-6. doi: 10.1111/j.1445-5994.1985.tb02761.x.
A radioimmunoassay for bovine osteocalcin has been developed. Human osteocalcin reacted identically with the bovine standard, allowing the use of this assay to measure human plasma osteocalcin. Levels were determined in 212 healthy subjects (124 men, 88 women) with an age range of 20 to 66 years. The distribution of these was skewed to the right, with the mean being 14.7 ng/ml (range 4 to 40) and the geometric mean 12.2 ng/ml. There was no alteration with age and no difference between males and females. High levels were found in chronic renal failure, Paget's disease of bone, and in primary hyperparathyroidism with severe bone disease, and there was a significant positive correlation of osteocalcin with plasma alkaline phosphatase. Low levels were found in pregnancy. Evidence is presented which suggests that the high levels measured probably reflect intact osteocalcin and not immunoreactive fragments. Our data are compared with those reported by others. Areas of disagreement are noted and discussed.
已开发出一种用于检测牛骨钙素的放射免疫分析法。人骨钙素与牛标准品反应相同，因此可使用该分析法来检测人血浆骨钙素。对年龄在20至66岁之间的212名健康受试者（124名男性，88名女性）进行了检测。其分布呈右偏态，均值为14.7 ng/ml（范围为4至40），几何均值为12.2 ng/ml。未发现随年龄变化的情况，男性和女性之间也无差异。在慢性肾衰竭、骨Paget病以及伴有严重骨病的原发性甲状旁腺功能亢进症中发现骨钙素水平较高，且骨钙素与血浆碱性磷酸酶呈显著正相关。在孕期发现骨钙素水平较低。有证据表明所测得的高水平可能反映的是完整的骨钙素而非免疫反应性片段。我们的数据与其他研究者报告的数据进行了比较。指出并讨论了存在分歧的方面。
