Centre of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
LABBELS─Associate Laboratory, Braga 4710-057, Portugal.
ACS Synth Biol. 2024 Jun 21;13(6):1727-1736. doi: 10.1021/acssynbio.4c00059. Epub 2024 May 24.
Curcumin, a natural polyphenol derived from turmeric, has attracted immense interest due to its diverse pharmacological properties. Traditional extraction methods from plants present limitations in meeting the growing demand for this bioactive compound, giving significance to its production by genetically modified microorganisms. Herein, we have developed an engineered to produce curcumin from glucose. A pathway composed of the 4-hydroxyphenylacetate 3-monooxygenase oxygenase complex from and , caffeic acid -methyltransferase from , feruloyl-CoA synthetase from , and diketide-CoA synthase and curcumin synthase from was introduced in a coumaric acid overproducing strain. This strain produced 240.1 ± 15.1 μg/L of curcumin. Following optimization of phenylpropanoids conversion, a strain capable of producing 4.2 ± 0.6 mg/L was obtained. This study reports for the first time the successful production of curcumin in .
姜黄素是一种天然多酚类物质，来源于姜黄，因其多种药理学特性而备受关注。传统的植物提取方法在满足对这种生物活性化合物日益增长的需求方面存在局限性，因此通过基因修饰微生物生产姜黄素具有重要意义。在此，我们构建了一个工程菌，用于从葡萄糖生产姜黄素。该途径由 4- 羟苯基乙酸 3-单加氧酶氧合酶复合物、来自 的咖啡酸 -O-甲基转移酶、来自 的阿魏酰辅酶 A 合成酶、来自 的二酮酸 -CoA 合酶和来自 的姜黄素合酶组成，在一个香豆酸过量产生的 菌株中进行了导入。该菌株产生了 240.1 ± 15.1 μg/L 的姜黄素。在优化苯丙烷类化合物转化后，获得了能够生产 4.2 ± 0.6 mg/L 姜黄素的菌株。本研究首次报道了在 中成功生产姜黄素。
