Wick G, Hönigsmann H, Timpl R
J Invest Dermatol. 1979 Nov;73(5):335-8. doi: 10.1111/1523-1747.ep12550349.
Specific rabbit antibodies to type IV collagen isolated from a basement membrane producing mouse tumor were used in indirect immunofluorescence tests to study the thickened vascular basement membrane in skin biopsies from patients with erythropoietic porphyria (EPP) and from protoporphyric mice. In addition, rabbit antibodies to a noncollagenous, basement membrane specific glycoprotein also derived from the mouse tumor were tested. It was shown that normal as well as altered vascular basement membranes in both the human and the murine skin specimens react with the anti-type IV collagen and the antiglycoprotein antibodies. A particular strong reaction in the diseases skin indicated that formation of new vascular basement membrane layers involved deposition of the major structural proteins which also constitute normal basement membrane matrices.
从产生基底膜的小鼠肿瘤中分离出的抗IV型胶原特异性兔抗体，被用于间接免疫荧光试验，以研究红细胞生成性卟啉病（EPP）患者及原卟啉小鼠皮肤活检中增厚的血管基底膜。此外，还检测了同样源自该小鼠肿瘤的一种非胶原性、基底膜特异性糖蛋白的兔抗体。结果显示，人类和小鼠皮肤标本中的正常及改变的血管基底膜均与抗IV型胶原抗体和抗糖蛋白抗体发生反应。疾病皮肤中出现的特别强烈的反应表明，新血管基底膜层的形成涉及到主要结构蛋白的沉积，这些蛋白也构成正常的基底膜基质。
