Li Yangyang, Cui Lin, Zhang Yutong, Zheng Jiahui, Ai Jinxia
School of Medical Technology, Beihua University, Jilin 132013, China.
School of Medical Technology, Beihua University, Jilin 132013, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2024 May;40(5):428-434.
Objective To establish a colloidal gold immunochromatography and develop the corresponding test strip for detecting organophosphorus compounds including omethoate, phoxim, dipterex, and parathion in fruits, vegetables and drinking water. Methods Artificial antigen molecules of organophosphorus compounds were synthesized using N-hydroxysuccinimide esters. Acetylcholinesterase antigen was prepared and purified, and the serum containing the corresponding antibody was prepared, purified, and labeled. The working parameters of the test strip were optimized, and the performance evaluation of it was conducted. Results The titer of the antisera ranged from 1:32 to 1:64, with a protein content of approximately 2 mg/mL. The purified polyclonal antibodies displayed target bands at relative molecular masses (M) of 25 000 and 55 000, indicating satisfactory purity. The reaction time of the test strips was between 5 to 10 minutes, with a detection limit for samples at 200 ng/mL. Both specificity and accuracy were satisfactory, and the test strip remained valid for 6 months. Conclusion A simple and rapid colloidal gold immunochromatography is established successfully for detecting several organophosphorus compounds and may be useful for on-site preliminary screening of samples in large quantities.
目的 建立一种胶体金免疫层析法并开发相应的检测试纸条，用于检测水果、蔬菜及饮用水中的有机磷化合物，包括氧乐果、辛硫磷、敌百虫和对硫磷。方法 采用N-羟基琥珀酰亚胺酯合成有机磷化合物的人工抗原分子。制备并纯化乙酰胆碱酯酶抗原，制备、纯化并标记含相应抗体的血清。优化试纸条的工作参数，并对其进行性能评价。结果 抗血清效价为1:32至1:64，蛋白质含量约为2 mg/mL。纯化的多克隆抗体在相对分子质量（M）为25 000和55 000处显示目标条带，表明纯度良好。试纸条的反应时间为5至10分钟，样品检测限为200 ng/mL。特异性和准确性均令人满意，试纸条有效期为6个月。结论 成功建立了一种简单快速的胶体金免疫层析法，用于检测多种有机磷化合物，可用于大量样品的现场初步筛查。
