Fisheries College, Zhejiang Ocean University, Zhoushan 316022, China.
Fisheries College, Jimei University, Xiamen 361021, China.
Genes (Basel). 2024 Apr 27;15(5):557. doi: 10.3390/genes15050557.
The systematic revision of the family Peristediidae remains an unresolved issue due to their diverse and unique morphology. Despite the popularity of using mitochondrial genome research to comprehensively understand phylogenetic relationships in fish, genetic data for peristediid fish need to be included. Therefore, this study aims to investigate the mitochondrial genomic characteristics and intra-family phylogenetic relationships of Peristediidae by utilizing mitochondrial genome analysis. Therefore, this study aims to investigate the phylogenetic relationship of Peristediidae by utilizing mitochondrial genome analysis. The mitochondrial genome of four species of Peristediidae (, , , and ) collected in the East China Sea was studied. The mitochondrial gene sequence lengths of four fish species were 16,533 bp, 16,526 bp, 16,527 bp, and 16,526 bp, respectively. They had the same mitochondrial structure and were all composed of 37 genes and one control region. Most PCGs used ATG as the start codon, and a few used GTG as the start codon. An incomplete stop codon (TA/T) occurred. The AT-skew and GC-skew values of 13 PCGs from four species were negative, and the GC-skew amplitude was greater than that of AT-skew. All cases of D-arm were found in (GCT). The Ka/Ks ratio analysis indicated that 13 PCGs were suffering purifying selection. Based on 12 PCGs (excluding ND6) sequences, a phylogenetic tree was constructed using Bayesian inference (BI) and maximum likelihood (ML) methods, providing a further supplement to the scientific classification of Peristediidae fish. According to the results of divergence time, the four species of fish had apparent divergence in the Early Cenozoic, which indicates that the geological events at that time caused the climax of species divergence and evolution.
由于 Peristediidae 家族的形态多样且独特，因此对其进行系统修订仍然是一个悬而未决的问题。尽管使用线粒体基因组研究来全面了解鱼类的系统发育关系已经很普遍，但仍需要包括 Peristediid 鱼类的遗传数据。因此，本研究旨在利用线粒体基因组分析研究 Peristediidae 的线粒体基因组特征和家族内的系统发育关系。因此，本研究旨在利用线粒体基因组分析研究 Peristediidae 的系统发育关系。本研究对东海采集的 4 种 Peristediidae（、、、和）的线粒体基因组进行了研究。4 种鱼类的线粒体基因序列长度分别为 16533bp、16526bp、16527bp 和 16526bp，它们具有相同的线粒体结构，均由 37 个基因和一个控制区组成。大多数 PCGs 使用 ATG 作为起始密码子，少数使用 GTG 作为起始密码子。出现了不完全终止密码子（TA/T）。4 种鱼类的 13 个 PCGs 的 AT-skew 和 GC-skew 值均为负值，GC-skew 幅度大于 AT-skew。所有 D-臂均在 （GCT）中发现。Ka/Ks 比值分析表明，13 个 PCGs 受到纯化选择。基于 12 个 PCGs（不包括 ND6）序列，使用贝叶斯推断（BI）和最大似然（ML）方法构建了系统发育树，为 Peristediidae 鱼类的科学分类提供了进一步的补充。根据分化时间的结果，这 4 种鱼类在早新生代明显分化，表明当时的地质事件导致了物种分化和进化的高潮。
