Arribas Victor, Monteoliva Lucia, Hernáez María Luisa, Gil Concha, Molero Gloria
University of Salamanca (USAL), 37008 Salamanca, Spain.
Department of Microbiology and Parasitology, Faculty of Pharmacy, Complutense University of Madrid (UCM), 28040 Madrid, Spain.
Antioxidants (Basel). 2024 Apr 26;13(5):527. doi: 10.3390/antiox13050527.
Prn1 is a protein with an unknown function similar to mammalian Pirin. It also has orthologues in other pathogenic fungi, but not in . Prn1 highly increases its abundance in response to HO treatment; thus, to study its involvement in the oxidative stress response, a mutant and the corresponding wild-type strain SN250 have been studied. Under HO treatment, Prn1 absence led to a higher level of reactive oxygen species (ROS) and a lower survival rate, with a higher percentage of death by apoptosis, confirming its relevant role in oxidative detoxication. The quantitative differential proteomics studies of both strains in the presence and absence of HO indicated a lower increase in proteins with oxidoreductase activity after the treatment in the strain, as well as an increase in proteasome-activating proteins, corroborated by in vivo measurements of proteasome activity, with respect to the wild type. In addition, remarkable differences in the abundance of some transcription factors were observed between mutant and wild-type strains, e.g., Mnl1 or Nrg1, an Mnl1 antagonist. orf19.4850, a protein orthologue to Cub1, has shown its involvement in the response to HO and in proteasome function when Prn1 is highly expressed in the wild type.
Prn1是一种功能未知的蛋白质，类似于哺乳动物的吡啉蛋白。它在其他致病真菌中也有直系同源物，但在[此处原文缺失相关信息]中没有。Prn1在过氧化氢（HO）处理后其丰度会显著增加；因此，为了研究其在氧化应激反应中的作用，对一个突变体和相应的野生型菌株SN250进行了研究。在HO处理下，Prn1缺失导致活性氧（ROS）水平升高和存活率降低，凋亡死亡的百分比更高，证实了其在氧化解毒中的相关作用。对这两种菌株在有和没有HO的情况下进行的定量差异蛋白质组学研究表明，与野生型相比，突变体菌株在处理后具有氧化还原酶活性的蛋白质增加较少，同时蛋白酶体激活蛋白增加，这一点通过蛋白酶体活性的体内测量得到了证实。此外，在突变体和野生型菌株之间观察到一些转录因子丰度的显著差异，例如Mnl1或Nrg1（Mnl1的拮抗剂）。orf19.4850是Cub1的蛋白质直系同源物，当Prn1在野生型中高度表达时，它已显示出参与对HO的反应和蛋白酶体功能。
