State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, China.
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2024 May 14;25(10):5346. doi: 10.3390/ijms25105346.
A homeobox transcription factor is a conserved transcription factor, ubiquitous in eukaryotes, that regulates the tissue formation of structure, cell differentiation, proliferation, and cancer. This study identified the homeobox transcription factor family and its distribution in var. at the whole genome level. It elucidated the gene structures and evolutionary characteristics of this family. Additionally, knockout experiments were carried out and the preliminary function of these transcription factors was studied. Through bioinformatics approaches, nine homeobox transcription factors () were identified in var. , and these contained HOX-conserved domains and helix-turn-helix secondary structures. Nine homeobox gene deletion mutants were obtained using the homologous recombinant gene knockout technique. Protoplast transformation was mediated by polyethylene glycol (PEG) and the transformants were identified using PCR. The knockouts of , , , , , , and genes resulted in a smaller growth diameter in var. . In contrast, the knockouts of the , , and genes inhibited the formation of conidia and led to a significant decrease in the pathogenicity. This study's results will provide insights for understanding the growth and development of var. . The pathogenic mechanism of the affected sugarcane will provide an essential theoretical basis for preventing and controlling sugarcane twisted leaf disease.
一个同源盒转录因子是一种保守的转录因子，在真核生物中普遍存在，它调节结构的组织形成、细胞分化、增殖和癌症。本研究在全基因组水平上鉴定了 var. 中的同源盒转录因子家族及其分布。它阐明了这个家族的基因结构和进化特征。此外，还进行了敲除实验，研究了这些转录因子的初步功能。通过生物信息学方法，在 var. 中鉴定了 9 个同源盒转录因子 ()，这些转录因子包含 HOX 保守结构域和螺旋-转角-螺旋二级结构。利用同源重组基因敲除技术获得了 9 个同源盒基因缺失突变体。原生质体转化通过聚乙二醇 (PEG) 介导，并通过 PCR 鉴定转化体。基因的敲除导致 var. 的生长直径变小。相比之下，基因的敲除抑制了分生孢子的形成，导致致病性显著下降。本研究的结果将为理解 var. 的生长和发育提供见解。受影响甘蔗的致病机制将为防治甘蔗扭叶病提供重要的理论基础。
