College of Landscape Architecture and Arts, Northwest A&F University, Yangling District, Xianyang 712100, China.
Oil Peony Engineering Technology Research Center of National Forestry Administration, Yangling District, Xianyang 712100, China.
Int J Mol Sci. 2024 May 17;25(10):5462. doi: 10.3390/ijms25105462.
is an important economic oil and medicinal crop. Its anthers are often used to make tea in China with beneficial effects on human health. However, the metabolite profiles, as well as potential biological activities of anthers and the pollen within anthers have not been systematically analyzed, which hinders the improvement of utilization. With comprehensive untargeted metabolomic analysis using UPLC-QTOF-MS, we identified a total of 105 metabolites in anthers and pollen, mainly including phenylpropanoids, polyketides, organic acids, benzenoids, lipids, and organic oxygen compounds. Multivariate statistical analysis revealed the metabolite differences between anthers and pollen, with higher carbohydrates and flavonoids content in pollen and higher phenolic content in anthers. Meanwhile, both anthers and pollen extracts exhibited antioxidant activity, antibacterial activity, α-glucosidase and α-amylase inhibitory activity. In general, the anther stage of S4 showed the highest biological activity among all samples. This study illuminated the metabolites and biological activities of anthers and pollen of , which supports the further utilization of them.
是一种重要的经济油料作物和药用作物。其花药在中国常被用来泡茶，对人体健康有益。然而，花药和花药内花粉的代谢物谱以及潜在的生物活性尚未得到系统分析，这阻碍了其利用的提高。本研究采用 UPLC-QTOF-MS 进行全面的非靶向代谢组学分析，共鉴定出 105 种花药和花粉中的代谢物，主要包括苯丙烷类、多酮类、有机酸类、苯类、脂类和有机含氧化合物。多变量统计分析揭示了花药和花粉之间的代谢物差异，花粉中碳水化合物和类黄酮含量较高，而花药中酚类物质含量较高。同时，花药和花粉提取物均表现出抗氧化活性、抗菌活性、α-葡萄糖苷酶和α-淀粉酶抑制活性。总体而言，所有样本中，S4 期的花药阶段表现出最高的生物活性。本研究阐明了 的花药和花粉的代谢物和生物活性，支持了它们的进一步利用。
