用深共晶溶剂从 Rehder & E.H.Wilson 残余生物质中提取木脂素类化合物及其生物活性。

Extraction and Biological Activity of Lignanoids from Rehder & E.H.Wilson Residual Waste Biomass Using Deep Eutectic Solvents.

机构信息

College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China.

Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, Yongzhou 425199, China.

出版信息

Molecules. 2024 May 16;29(10):2352. doi: 10.3390/molecules29102352.

DOI:10.3390/molecules29102352

PMID:38792212

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11124428/

Abstract

Lignanoids are an active ingredient exerting powerful antioxidant and anti-inflammatory effects in the treatment of many diseases. In order to improve the efficiency of the resource utilization of traditional Chinese medicine waste, Rehder & E.H.Wilson residue (MOR) waste biomass was used as raw material in this study, and a series of deep eutectic solvents (ChUre, ChAce, ChPro, ChCit, ChOxa, ChMal, ChLac, ChLev, ChGly and ChEG) were selected to evaluate the extraction efficiency of lignanoids from MORs. The results showed that the best conditions for lignanoid extraction were a liquid-solid ratio of 40.50 mL/g, an HBD-HBA ratio of 2.06, a water percentage of 29.3%, an extract temperature of 337.65 K, and a time of 107 min. Under these conditions, the maximum lignanoid amount was 39.18 mg/g. In addition, the kinetics of the extraction process were investigated by mathematic modeling. In our antioxidant activity study, high antioxidant activity of the lignanoid extract was shown in scavenging four different types of free radicals (DPPH, ·OH, ABTS, and superoxide anions). At a concentration of 3 mg/mL, the total antioxidant capacity of the lignanoid extract was 1.795 U/mL, which was equal to 0.12 mg/mL of V solution. Furthermore, the antibacterial activity study found that the lignanoid extract exhibited good antibacterial effects against six tested pathogens. Among them, exerted the strongest antibacterial activity. Eventually, the correlation of the lignanoid extract with the biological activity and physicochemical properties of DESs is described using a heatmap, along with the evaluation of the in vitro hypoglycemic, in vitro hypolipidemic, immunomodulatory, and anti-inflammatory activity of the lignanoid extract. These findings can provide a theoretical foundation for the extraction of high-value components from waste biomass by deep eutectic solvents, as well as highlighting its specific significance in natural product development and utilization.

摘要

木脂素是一种具有强大抗氧化和抗炎作用的活性成分，可用于治疗多种疾病。为了提高中药废渣资源的利用效率，本研究以瑞香科狼毒属植物 Rehder & E.H.Wilson 废渣（MOR）为原料，选用一系列深共晶溶剂（ChUre、ChAce、ChPro、ChCit、ChOxa、ChMal、ChLac、ChLev、ChGly 和 ChEG）评估 MOR 中木脂素的提取效率。结果表明，木脂素提取的最佳条件为液固比 40.50 mL/g、HBD-HBA 比 2.06、水含量 29.3%、提取温度 337.65 K、时间 107 min。在此条件下，木脂素的最大含量为 39.18 mg/g。此外，通过数学建模研究了提取过程的动力学。在抗氧化活性研究中，木脂素提取物对四种不同类型自由基（DPPH、·OH、ABTS 和超氧阴离子）具有高抗氧化活性。在 3 mg/mL 浓度下，木脂素提取物的总抗氧化能力为 1.795 U/mL，与 0.12 mg/mL V 溶液相当。此外，抗菌活性研究发现，木脂素提取物对 6 种测试病原体具有良好的抗菌作用。其中，对革兰氏阳性菌的抑制作用最强。最后，采用热图描述了木脂素提取物与 DES 的生物活性和物理化学性质的相关性，并评价了木脂素提取物的体外降血糖、体外降血脂、免疫调节和抗炎活性。这些发现为利用深共晶溶剂从废渣中提取高价值成分提供了理论基础，并突出了其在天然产物开发利用中的特殊意义。