Unité de Brasserie et des Industries Alimentaires, Louvain Institute of Biomolecular Science and Technology (LIBST), Faculté des Bioingénieurs, Université Catholique de Louvain, Croix du Sud, 2 Box L7.05.07, B-1348 Louvain-la-Neuve, Belgium.
Molecules. 2024 May 17;29(10):2370. doi: 10.3390/molecules29102370.
Even when fresh, non-alcoholic, and low-alcoholic beers (NABLABs) exhibit significant staling defects due to premature oxidation. In this study, the antioxidant power of eleven fresh commercial NABLABs was assessed by means of three different assays: the oxygen radical absorbance capacity (ORAC), the linoleic acid-induced oxidation (TINH), and the indicator time test (ITT). Only the first two assays, both involving radicalar degradations initiated by AAPH, were found to correlate with each other. NABLABs displayed lower ORAC values than conventional beers (on average, 6127 μmol eq. Trolox/L), except for three samples made with special-colored malts or dry-hopped. Dealcoholization was the step with the greatest impact on the ORAC value (up to a 95% loss) and on flavan-3-ols, sotolon, and polyfunctional thiols, while pasteurization strongly affected color, TBA, and Strecker aldehydes. ORAC assays applied to hop, alternative cereals, and various botanical ingredients indicated that mashing with red sorghum, dry hopping/spicing, and wood maturation could bring the antioxidant power of a NABLAB close to those of conventional beers. With an ORAC value not reached by any other tested botanical ingredient (5234 µmol eq. Trolox/g), African leaves (traditionally used for Rwandan Ikigage beers) emerged here as the best candidate.
即使是新鲜的、无酒精和低酒精啤酒(NABLABs)也会由于过早氧化而表现出明显的陈化缺陷。在这项研究中,通过三种不同的测定方法评估了 11 种新鲜商业 NABLABs 的抗氧化能力:氧自由基吸收能力(ORAC)、亚油酸诱导氧化(TINH)和指示剂时间测试(ITT)。只有前两种测定方法(均涉及 AAPH 引发的自由基降解)被发现彼此相关。NABLABs 的 ORAC 值低于传统啤酒(平均为 6127 μmol eq. Trolox/L),除了三种使用特殊颜色麦芽或干投酿造的样品。脱醇是对 ORAC 值(高达 95%的损失)和黄烷-3-醇、索特隆和多功能硫醇影响最大的步骤,而巴氏杀菌强烈影响颜色、TBA 和 Strecker 醛。应用于啤酒花、替代谷物和各种植物成分的 ORAC 测定表明,用红高粱糖化、干投/调味和木材陈化可以使 NABLAB 的抗氧化能力接近传统啤酒。一种植物成分(5234 µmol eq. Trolox/g)的 ORAC 值未达到,非洲叶子(传统上用于卢旺达 Ikigage 啤酒)在这里成为最佳候选者。