• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

功能化离子液体修饰的金属有机骨架材料提高脂肪酶的酶催化性能。

Functionalized Ionic Liquids-Modified Metal-Organic Framework Material Boosted the Enzymatic Performance of Lipase.

机构信息

State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China.

出版信息

Molecules. 2024 May 18;29(10):2381. doi: 10.3390/molecules29102381.

DOI:10.3390/molecules29102381
PMID:38792242
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11124374/
Abstract

The development of immobilized enzymes with high activity and stability is critical. Metal-organic frameworks (MOFs) have attracted much academic and industrial interest in the field of enzyme immobilization due to their unique properties. In this study, the amino-functionalized ionic liquid (NIL)-modified metal-organic framework (UiO-66-NH) was prepared to immobilize lipase (CRL), using dialdehyde starch (DAS) as the cross-linker. The results of the Fourier transform infrared (FT-IR) spectra, X-ray powder diffraction (XRD), and scanning electronic microscopy (SEM) confirmed that the NIL was successfully grafted to UiO-66-NH. The CRL immobilized on NIL-modified UiO-66-NH (UiO-66-NH-NIL-DAS@CRL) exhibited satisfactory activity recovery (79.33%), stability, reusability, and excellent organic solvent tolerance. The research results indicated that ionic liquid-modified UiO-66-NH had practical potential for application in enzyme immobilization.

摘要

固定化酶的高活性和稳定性的发展至关重要。由于其独特的性质，金属有机骨架（MOFs）在酶固定化领域引起了学术界和工业界的极大兴趣。在这项研究中，制备了氨基功能化离子液体（NIL）修饰的金属有机骨架（UiO-66-NH），并使用二醛淀粉（DAS）作为交联剂固定脂肪酶（CRL）。傅里叶变换红外（FT-IR）光谱、X 射线粉末衍射（XRD）和扫描电子显微镜（SEM）的结果证实，NIL 成功地接枝到了 UiO-66-NH 上。固定在 NIL 修饰的 UiO-66-NH（UiO-66-NH-NIL-DAS@CRL）上的 CRL 表现出令人满意的活性回收率（79.33%）、稳定性、可重复使用性和出色的有机溶剂耐受性。研究结果表明，离子液体修饰的 UiO-66-NH 在酶固定化方面具有实际应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/a5ccfdd5e3cd/molecules-29-02381-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/24627a24dbf1/molecules-29-02381-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/8cb35186ac30/molecules-29-02381-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/98c78355129e/molecules-29-02381-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/7b8526a03ae0/molecules-29-02381-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/35401a49b813/molecules-29-02381-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/ce6fadc52958/molecules-29-02381-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/45305bea06bb/molecules-29-02381-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/bbe113ae32e0/molecules-29-02381-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/a5ccfdd5e3cd/molecules-29-02381-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/24627a24dbf1/molecules-29-02381-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/8cb35186ac30/molecules-29-02381-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/98c78355129e/molecules-29-02381-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/7b8526a03ae0/molecules-29-02381-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/35401a49b813/molecules-29-02381-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/ce6fadc52958/molecules-29-02381-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/45305bea06bb/molecules-29-02381-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/bbe113ae32e0/molecules-29-02381-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a89/11124374/a5ccfdd5e3cd/molecules-29-02381-g008.jpg

相似文献

1
Functionalized Ionic Liquids-Modified Metal-Organic Framework Material Boosted the Enzymatic Performance of Lipase.功能化离子液体修饰的金属有机骨架材料提高脂肪酶的酶催化性能。
Molecules. 2024 May 18;29(10):2381. doi: 10.3390/molecules29102381.
2
Preparation and Characterization of Magnetic Metal-Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase.磁性金属有机框架的制备及离子液体功能化表征作为固定化脂肪酶载体。
Molecules. 2022 Oct 11;27(20):6800. doi: 10.3390/molecules27206800.
3
Surfactant-based metal-organic frameworks (MOFs) in the preparation of an active biocatalysis.基于表面活性剂的金属有机骨架（MOFs）在活性生物催化中的制备。
J Biotechnol. 2023 Jul 20;371-372:10-21. doi: 10.1016/j.jbiotec.2023.06.003. Epub 2023 Jun 8.
4
Metal-Organic Frameworks Conjugated Lipase with Enhanced Bio-catalytic Activity and Stability.金属有机框架与脂肪酶共轭，提高生物催化活性和稳定性。
Appl Biochem Biotechnol. 2020 Sep;192(1):132-145. doi: 10.1007/s12010-020-03268-z. Epub 2020 Apr 22.
5
Proline-Modified UIO-66 as Nanocarriers to Enhance Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen.脯氨酸修饰的 UIO-66 作为纳米载体，提高脂肪酶的催化活性和稳定性，用于电化学检测硝基酚。
ACS Appl Mater Interfaces. 2021 Jan 27;13(3):4146-4155. doi: 10.1021/acsami.0c17134. Epub 2021 Jan 13.
6
Immobilization of Lipase by Ionic Liquid-Modified Mesoporous SiO Adsorption and Calcium Alginate-Embedding Method.离子液体修饰介孔 SiO2 吸附和海藻酸钙包埋法固定化脂肪酶。
Appl Biochem Biotechnol. 2018 Jul;185(3):606-618. doi: 10.1007/s12010-017-2676-0. Epub 2017 Dec 16.
7
Assessment of the efficiency and stability of enzymatic membrane reaction utilizing lipase covalently immobilized on a functionalized hybrid membrane.利用固定在功能化杂化膜上的脂肪酶进行酶膜反应的效率和稳定性评估。
J Biotechnol. 2024 May 20;387:23-31. doi: 10.1016/j.jbiotec.2024.03.012. Epub 2024 Mar 26.
8
Enhancing bio-catalytic activity and stability of lipase nanogel by functional ionic liquids modification.通过功能化离子液体修饰提高脂肪酶纳米凝胶的生物催化活性和稳定性。
Colloids Surf B Biointerfaces. 2020 Nov;195:111275. doi: 10.1016/j.colsurfb.2020.111275. Epub 2020 Jul 24.
9
Calix[4]arene tetracarboxylic acid-treated lipase immobilized onto metal-organic framework: Biocatalyst for ester hydrolysis and kinetic resolution.杯[4]芳烃四羧酸处理的固定化脂肪酶：用于酯水解和动力学拆分的生物催化剂。
Int J Biol Macromol. 2021 Apr 1;175:79-86. doi: 10.1016/j.ijbiomac.2021.02.003. Epub 2021 Feb 4.
10
Covalent immobilization of lipase on an ionic liquid-functionalized magnetic Cu-based metal-organic framework with boosted catalytic performance in flavor ester synthesis.离子液体功能化磁性 Cu 基金属有机骨架上脂肪酶的共价固定及其在风味酯合成中催化性能的增强。
J Mater Chem B. 2023 Feb 8;11(6):1302-1311. doi: 10.1039/d2tb02246j.

引用本文的文献

1
Metal-organic framework-mediated antioxidant enzyme delivery in disease treatment.金属有机框架介导的抗氧化酶递送在疾病治疗中的应用
Redox Biol. 2025 Jul 18;85:103778. doi: 10.1016/j.redox.2025.103778.
2
Exploring the Biological Activities of Ionic Liquids and Their Potential to Develop Novel Vaccine Adjuvants.探索离子液体的生物活性及其开发新型疫苗佐剂的潜力。
Vaccines (Basel). 2025 Mar 28;13(4):365. doi: 10.3390/vaccines13040365.

本文引用的文献

1
Surface Modification of Magnetic ZIF-90 Nanoparticles Improves the Microenvironment of Immobilized Lipase and Its Application in Esterification.磁性ZIF-90纳米颗粒的表面改性改善了固定化脂肪酶的微环境及其在酯化反应中的应用。
Langmuir. 2022 Dec 13;38(49):15384-15393. doi: 10.1021/acs.langmuir.2c02672. Epub 2022 Nov 30.
2
Preparation and Characterization of Magnetic Metal-Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase.磁性金属有机框架的制备及离子液体功能化表征作为固定化脂肪酶载体。
Molecules. 2022 Oct 11;27(20):6800. doi: 10.3390/molecules27206800.
3
Immobilization of Interfacial Activated Lipase Onto Magnetic Chitosan Using Dialdehyde Cellulose as Cross-Linking Agent.
以二醛纤维素为交联剂将界面活化脂肪酶固定在磁性壳聚糖上
Front Bioeng Biotechnol. 2022 Jul 18;10:946117. doi: 10.3389/fbioe.2022.946117. eCollection 2022.
4
Enhancing Enzyme Activity by the Modulation of Covalent Interactions in the Confined Channels of Covalent Organic Frameworks.通过在共价有机框架的受限通道中调节共价相互作用来增强酶活性。
Angew Chem Int Ed Engl. 2022 May 16;61(21):e202201378. doi: 10.1002/anie.202201378. Epub 2022 Mar 24.
5
Reprogramming microbial populations using a programmed lysis system to improve chemical production.利用编程裂解系统重新编程微生物种群以提高化学品产量。
Nat Commun. 2021 Nov 25;12(1):6886. doi: 10.1038/s41467-021-27226-3.
6
Metal-Organic Framework-Based Enzyme Biocomposites.基于金属有机骨架的酶生物复合材料。
Chem Rev. 2021 Feb 10;121(3):1077-1129. doi: 10.1021/acs.chemrev.0c01029. Epub 2021 Jan 13.
7
Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts.聚乙烯亚胺：一种在固定化酶生物催化剂设计中非常有用的离子聚合物。
J Mater Chem B. 2017 Sep 28;5(36):7461-7490. doi: 10.1039/c7tb01639e. Epub 2017 Sep 4.
8
Programmable biomolecular switches for rewiring flux in Escherichia coli.可编程生物分子开关用于重新布线大肠杆菌中的通量。
Nat Commun. 2019 Aug 21;10(1):3751. doi: 10.1038/s41467-019-11793-7.
9
Enzymatic esterification of oleic acid by Candida rugosa lipase immobilized onto biochar.固定化在生物炭上的假丝酵母脂肪酶对油酸的酶促酯化作用。
J Environ Manage. 2019 Jul 15;242:171-177. doi: 10.1016/j.jenvman.2019.04.013. Epub 2019 May 3.
10
Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions.脂肪酶在疏水性载体上的固定化：固定化机制、优点、问题及解决方法。
Biotechnol Adv. 2019 Sep-Oct;37(5):746-770. doi: 10.1016/j.biotechadv.2019.04.003. Epub 2019 Apr 8.