Schwager J, Hadji-Azimi I
Differentiation. 1985;30(1):29-34. doi: 10.1111/j.1432-0436.1985.tb00509.x.
Anti-IgM induced the proliferation of spleen lymphocytes of the amphibian Xenopus laevis as determined by 3H-thymidine uptake. The responding cells were B lymphocytes, since lymphocyte populations enriched in surface-Ig-positive cells exhibited an increased proliferative response, and spleen cells from larvally thymectomized animals still responded to anti-IgM. Immunofluorescence analysis and gel electrophoresis of biosynthetically labeled Ig polypeptides revealed that lymphoblasts induced by anti-IgM differentiated into plasmablasts that synthesized and secreted mainly IgM and small amounts of IgY. The in vitro differentiation of B lymphocytes also occurred in spleen cells obtained from thymectomized animals. These findings are in contrast with those obtained in mammals and suggest that the differentiation of B lymphocytes in X. laevis is subject to different regulatory mechanisms.
通过³H-胸腺嘧啶核苷摄取测定，抗IgM可诱导两栖动物非洲爪蟾脾脏淋巴细胞增殖。应答细胞为B淋巴细胞，因为富含表面Ig阳性细胞的淋巴细胞群体表现出增强的增殖反应，并且来自幼虫期胸腺切除动物的脾脏细胞仍对抗IgM有反应。生物合成标记的Ig多肽的免疫荧光分析和凝胶电泳显示，抗IgM诱导的淋巴母细胞分化为主要合成和分泌IgM及少量IgY的成浆细胞。B淋巴细胞的体外分化也发生在胸腺切除动物的脾脏细胞中。这些发现与在哺乳动物中获得的结果相反，表明非洲爪蟾中B淋巴细胞的分化受不同的调节机制控制。
