Le Tuan Son, Southgate Paul C, O'Connor Wayne, Vu Sang V, Kurtböke D İpek
Research Institute for Marine Fisheries, 224 Le Lai, Ngo Quyen, Hai Phong 180000, Vietnam.
GeneCology Research Centre and School of Science and Engineering, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, Queensland 4556, Australia.
Antibiotics (Basel). 2020 Jul 16;9(7):415. doi: 10.3390/antibiotics9070415.
Mortalities of bivalve larvae and spat linked with spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bacteriophages is gaining interest in aquaculture as a more sustainable option for managing spp. infection. This study assessed the effectiveness of bacteriophages (Φ-5, Φ-6, and Φ-7) against pathogenic isolates (USC-26004 and USC-26005). These phage isolates were found to belong to the viral family. A total of 212 ORFs of Φ-5 were identified and annotated. The genome of this phage contained putative thymidine kinase and lysin enzyme. During infections with phages, the OD values of the isolates USC-26005 and USC-26004 remained stable at a much lower reading compared to the control after 9 h of incubation. Mortality rate of oyster () larvae was 28.2 ± 3.5% in the bacteriophage treatment group, compared to 77.9 ± 9.1% in the bacterial treatment group after 24 h incubation. Findings of this study indicate that lytic phages might be utilized as potential bio-control agents of luminescent bacterial disease in oyster hatcheries.
自1959年以来，双壳贝类幼虫和稚贝因感染弧菌属物种而死亡的情况在孵化场中已有记载，这导致了耐药细菌的潜在发展。针对这一问题，尚未开发出可靠且可持续的解决方案。利用噬菌体治疗细菌感染作为一种更可持续的管理弧菌属物种感染的选择，在水产养殖中越来越受到关注。本研究评估了噬菌体（Φ-5、Φ-6和Φ-7）对致病性弧菌分离株（USC-26004和USC-26005）的有效性。这些噬菌体分离株被发现属于弧菌病毒科。总共鉴定并注释了Φ-5的212个开放阅读框。该噬菌体的基因组包含推定的胸苷激酶和溶菌酶。在噬菌体感染期间，与对照相比，在孵育9小时后，分离株USC-26005和USC-26004的OD值在低得多的读数下保持稳定。在孵育24小时后，噬菌体处理组的牡蛎幼虫死亡率为28.2±3.5%，而细菌处理组为77.9±9.1%。本研究结果表明，裂解性噬菌体可能被用作牡蛎孵化场中发光细菌病的潜在生物控制剂。
