Beňo Filip, Velková Adéla, Hruška Filip, Ševčík Rudolf
Department of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Microorganisms. 2024 May 17;12(5):1010. doi: 10.3390/microorganisms12051010.
Lactoperoxidase (LP) is an important enzyme of the salivary and mammary glands. It has been proven to increase the shelf life of raw milk by inhibiting the growth of bacteria, especially , , , and spp. The aim of this work was to verify the use of LP to extend the shelf life of meat products. In vitro experiments showed inhibitory effects on the selected bacteria ( (ATCC 33090), (CP054440.1), and (ATCC 13525) due to a prolongation of the lag phase of growth curves. A lower increase in viable counts ( < 0.05) was also found by testing pork cubes' surface treated with LP solution (5%) + and stored for 7 days at 15 °C. LP has also been studied at concentrations of 0.25 and 0.50% in meat products (pork ham and pâté) during refrigerated storage (4 °C for 28 days). Lower viable counts were observed throughout the storage experiment, especially for 0.50% LP ( < 0.05). Meat products containing LP also showed lower levels of oxidation (MAD) ( < 0.05). According to these results, LP could extend the shelf life of a wider range of products.
乳过氧化物酶(LP)是唾液腺和乳腺中的一种重要酶。已证明它能通过抑制细菌生长来延长生乳的保质期,尤其是抑制、、和等菌属。本研究的目的是验证LP在延长肉制品保质期方面的应用。体外实验表明,由于生长曲线的滞后期延长,LP对所选细菌((美国典型培养物保藏中心33090)、(CP054440.1)和(美国典型培养物保藏中心13525))有抑制作用。在用LP溶液(5%)+处理过的猪肉块表面进行测试,并在15℃下储存7天后,还发现活菌数的增加较低(<0.05)。在冷藏储存(4℃,28天)期间,还对肉制品(猪肉火腿和肉酱)中0.25%和0.50%浓度的LP进行了研究。在整个储存实验中观察到活菌数较低,尤其是0.50%LP组(<0.05)。含有LP的肉制品也显示出较低的氧化水平(MAD)(<0.05)。根据这些结果,LP可以延长更广泛产品的保质期。