Oettler Martin J, Conraths Franz J, Roesler Uwe, Reiche Sven, Homeier-Bachmann Timo, Denzin Nicolai
Institute of Epidemiology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.
Institute for Animal Hygiene and Environmental Health, Freie Universität Berlin, 14163 Berlin, Germany.
Microorganisms. 2024 May 17;12(5):1019. doi: 10.3390/microorganisms12051019.
The aim of this study was to test the inactivation of viruses on germ carriers of different types of wood using a disinfectant in order to assess the biosafety of wood as a building material in animal husbandry. The laboratory disinfectant efficacy tests were based on German testing guidelines and current European standards. Five different types of wood germ carriers, i.e., spruce (), pine (), poplar ( sp.), beech () and Douglas fir (), were inoculated with enveloped or non-enveloped viruses and then treated with one of three different disinfectants. The results revealed that intact, fine-sawn timber with a low roughness depth can be effectively inactivated. Peracetic acid proved to be the most effective disinfectant across all tests. Regardless of the pathogen and the type of wood, a concentration of 0.1% of the pure substance at a temperature of 10 °C and an exposure time of one hour can be recommended. At a temperature of -10 °C, a concentration of 0.75% is recommended. The basic chemicals formic acid and glutaraldehyde demonstrated only limited effectiveness overall. The synergistic effects of various wood components on the inactivation of viruses offer potential for further investigation. Disinfectant tests should also be conclusively verified in field trials to ensure that the results from standardised laboratory tests can be transferred to real stable conditions.
本研究的目的是使用一种消毒剂测试不同类型木材的病菌载体上病毒的灭活情况，以便评估木材作为畜牧建筑材料的生物安全性。实验室消毒剂效果测试基于德国测试指南和现行欧洲标准。五种不同类型的木材病菌载体，即云杉、松木、杨树、山毛榉和花旗松，接种包膜或无包膜病毒，然后用三种不同消毒剂之一进行处理。结果表明，粗糙度深度低的完整细锯木材可有效灭活。在所有测试中，过氧乙酸被证明是最有效的消毒剂。无论病原体和木材类型如何，建议在10°C温度下，纯物质浓度为0.1%，暴露时间为1小时。在-10°C温度下，建议浓度为0.75%。基本化学物质甲酸和戊二醛总体上仅显示出有限的效果。各种木材成分对病毒灭活的协同作用为进一步研究提供了潜力。消毒剂测试还应在现场试验中进行最终验证，以确保标准化实验室测试的结果能够应用于实际的畜舍条件。
