Institute of Epidemiology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald, Germany.
Institute for Animal Hygiene and Environmental Health, Freie Universität Berlin, 14163 Berlin, Germany.
Viruses. 2024 May 15;16(5):789. doi: 10.3390/v16050789.
The aim of this study was to analyse the hygienic suitability of wood often used in animal husbandry. To this end, the inactivation of viruses (Enterovirus E as a surrogate for non-enveloped viruses and Newcastle disease virus as a surrogate for enveloped viruses) on germ carriers consisting of various types of wood was studied over an extended period to assess the biosafety of wood as an agricultural building material. The study was designed to assess the intrinsic biocidal activity of the wood itself, without the use of a disinfectant. The laboratory tests were based on German test guidelines and current European standards. Five different types of wood germ carriers, i.e., spruce (), pine (), poplar ( sp.), beech () and Douglas fir (), as well as stainless-steel carriers, were inoculated with enveloped and non-enveloped viruses and stored for up to four months, and the remaining infectivity of the viruses was continuously assessed. The results showed that intact, finely sawn timber with a low depth of roughness had an inactivating effect on the viruses up to 7.5 decadal logarithmic levels. For the non-enveloped virus, inactivation was fastest on Douglas fir wood, with the target reduction for effective inactivation (reduction by factor 4.0 log) being achieved after two weeks, and for the enveloped virus on pine wood, it was already achieved from the day of drying. The hygienic effects of the wood carriers may be due to their hygroscopic properties and wood constituents. These effects offer potential for further investigation, including tests with other wood species rich in extractives.
本研究旨在分析畜牧业常用木材的卫生适宜性。为此，研究了不同类型木材载体上病毒（肠道病毒 E 作为非包膜病毒的替代物，新城疫病毒作为包膜病毒的替代物）的失活情况，以评估木材作为农业建筑材料的生物安全性。该研究旨在评估木材本身的固有杀菌活性，而不使用消毒剂。实验室测试基于德国测试指南和当前的欧洲标准。使用了五种不同类型的木材载体，即云杉（）、松木（）、白杨（ sp.）、山毛榉（）和花旗松（），以及不锈钢载体，对包膜和非包膜病毒进行接种，并储存长达四个月，连续评估病毒的剩余感染力。结果表明，完整的、细锯的、粗糙度低的木材对病毒具有长达 7.5 个对数级的灭活作用。对于非包膜病毒，花旗松上的灭活速度最快，目标有效灭活减少（减少 4.0 对数）在两周后实现，而对于包膜病毒，在干燥的当天就已经实现。木材载体的卫生效果可能归因于其吸湿特性和木材成分。这些效果为进一步研究提供了潜力，包括对其他富含提取物的木材进行测试。
