Department of Orthopedics and Traumatology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400132 Cluj-Napoca, Romania.
Faculty of Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania.
Medicina (Kaunas). 2024 Apr 25;60(5):707. doi: 10.3390/medicina60050707.
: This scoping review investigates recent trends in adipose tissue-derived injectable therapies for osteoarthritis (OA) in animal models, focusing on minimally manipulated or lightly processed adipose tissue. By evaluating and examining the specific context in which these therapies were investigated across diverse animal OA models, this review aims to provide valuable insights that will inform and guide future research and clinical applications in the ongoing pursuit of effective treatments for osteoarthritis. : This research conducted a comprehensive literature review of PubMed and Embase to determine studies about minimally manipulated adipose tissue-derived injectable therapies for osteoarthritis investigated using animal models. The primary search found 530 results. After excluding articles that focused on spontaneous osteoarthritis; on transfected, preconditioned, cultured, or co-cultured adipose-derived stem cells; and articles with unavailable full text, we included 11 articles in our review. : The examined therapies encompassed mechanical micro-fragmented adipose tissue (MFAT) and stromal vascular fraction (SVF) obtained via collagenase digestion and centrifugation. These interventions were evaluated across various animal models, including mice, rats, rabbits, and sheep with induced OA. Notably, more studies concentrated on surgically induced OA rather than chemically induced OA. The assessment of these therapies focused on elucidating their protective immunomodulatory, anti-inflammatory, and chondroregenerative potential through comprehensive evaluations, including macroscopic assessments, histological analyses, immunohistochemical examinations, and biochemical assays. : This review provides a comprehensive analysis of adipose tissue-derived injectable therapies for osteoarthritis across diverse animal models. While revealing potential benefits and insights, the heterogeneity of data and the limited number of studies highlight the need for further research to formulate conclusive recommendations for clinical applications.
这篇范围界定综述调查了最近在动物模型中针对骨关节炎 (OA) 的脂肪组织衍生可注射疗法的趋势，重点关注经过最小处理或轻度加工的脂肪组织。通过评估和研究这些疗法在各种动物 OA 模型中的具体背景，本综述旨在提供有价值的见解，为正在进行的针对骨关节炎的有效治疗方法的研究和临床应用提供信息和指导。
这项研究对 PubMed 和 Embase 进行了全面的文献回顾，以确定使用动物模型研究经过最小处理的脂肪组织衍生可注射疗法治疗骨关节炎的研究。初步搜索发现了 530 个结果。在排除了专注于自发性骨关节炎的文章、转染的、预处理的、培养的或共培养的脂肪来源干细胞的文章以及无法获取全文的文章后，我们在综述中纳入了 11 篇文章。
研究中检查的疗法包括通过胶原酶消化和离心获得的机械微粉碎脂肪组织 (MFAT) 和基质血管部分 (SVF)。这些干预措施在各种动物模型中进行了评估，包括诱导 OA 的小鼠、大鼠、兔子和绵羊。值得注意的是，更多的研究集中在手术诱导的 OA 上，而不是化学诱导的 OA。这些疗法的评估侧重于通过全面评估，包括宏观评估、组织学分析、免疫组织化学检查和生化分析，阐明其保护免疫调节、抗炎和软骨再生潜力。
本综述全面分析了不同动物模型中脂肪组织衍生可注射疗法治疗骨关节炎的情况。尽管揭示了潜在的益处和见解，但数据的异质性和研究数量的有限性突出表明需要进一步研究，以便为临床应用制定明确的建议。
