Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA.
Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA.
Viruses. 2024 Apr 30;16(5):715. doi: 10.3390/v16050715.
In September 2021, 14 smallmouth bass (SMB; with skin lesions were collected from Green Bay waters of Lake Michigan and submitted for diagnostic evaluation. All the skin samples tested positive for largemouth bass virus (LMBV) by conventional PCR. The complete genome of the LMBV (99,328 bp) isolated from a homogenized skin sample was determined using an Illumina MiSeq sequencer. A maximum likelihood (ML) phylogenetic analysis based on the 21 core iridovirus genes supported the LMBV isolated from SMB (LMBV-WVL21117) as a member of the species . Pairwise nucleotide comparison of the major capsid protein (MCP) gene showed that LMBV-WVL21117 is identical to other LMBV reported from the United States and nearly identical to doctor fish virus and guppy virus 6 (99.2%) from Southeast Asia, as well as LMBV isolates from China and Thailand (99.1%). In addition, ML phylogenetic analysis based on the MCP gene suggests three genotypes of LMBV separated by region: genotype one from the United States, genotype two from Southeast Asia, and genotype three from China and Thailand. Additional research is needed to understand the prevalence and genetic diversity of LMBV strains circulating in wild and managed fish populations from different regions.
2021 年 9 月，从密歇根湖的绿湾水域采集了 14 条带有皮肤损伤的小口黑鲈（SMB），并提交进行诊断评估。所有皮肤样本均通过常规 PCR 检测出大口黑鲈病毒（LMBV）阳性。从均质皮肤样本中分离出的 LMBV（99328bp）的完整基因组使用 Illumina MiSeq 测序仪确定。基于 21 个核心虹彩病毒基因的最大似然（ML）系统发育分析支持从 SMB 分离出的 LMBV（LMBV-WVL21117）是种的成员。主要衣壳蛋白（MCP）基因的成对核苷酸比较表明，LMBV-WVL21117与美国其他报道的 LMBV 相同，与来自东南亚的医生鱼病毒和孔雀鱼病毒 6（99.2%）以及来自中国和泰国的 LMBV 几乎相同（99.1%）。此外，基于 MCP 基因的 ML 系统发育分析表明，LMBV 有三种基因型，按地区划分：一种来自美国，二种来自东南亚，三种来自中国和泰国。需要进一步研究来了解不同地区野生和养殖鱼类种群中循环的 LMBV 菌株的流行情况和遗传多样性。
