Li Linzhuo, Jiang Fuyan, Bi Yaqi, Yin Xingfu, Zhang Yudong, Li Shaoxiong, Zhang Xingjie, Liu Meichen, Li Jinfeng, Shaw Ranjan K, Ijaz Babar, Fan Xingming
Institute of Resource Plants, Yunnan University, Kunming 650500, China.
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Plants (Basel). 2024 May 18;13(10):1410. doi: 10.3390/plants13101410.
Common rust (CR), caused by , is a major foliar disease in maize that leads to quality deterioration and yield losses. To dissect the genetic architecture of CR resistance in maize, this study utilized the susceptible temperate inbred line Ye107 as the male parent crossed with three resistant tropical maize inbred lines (CML312, D39, and Y32) to generate 627 F recombinant inbred lines (RILs), with the aim of identifying maize disease-resistant loci and candidate genes for common rust. Phenotypic data showed good segregation between resistance and susceptibility, with varying degrees of resistance observed across different subpopulations. Significant genotype effects and genotype × environment interactions were observed, with heritability ranging from 85.7% to 92.2%. Linkage and genome-wide association analyses across the three environments identified 20 QTLs and 62 significant SNPs. Among these, seven major QTLs explained 66% of the phenotypic variance. Comparison with six SNPs repeatedly identified across different environments revealed overlap between and , and . Haplotype analysis indicated two different haplotypes for CR resistance for both the SNPs. Based on LD decay plots, three co-located candidate genes, , , and , were identified within 20 kb upstream and downstream of these two SNPs. regulates hormone regulation, controls stomatal opening and closure, related to trichome, and is associated with plant disease immune responses. Additionally, we performed candidate gene screening for five additional SNPs that were repeatedly detected across different environments, resulting in the identification of five candidate genes. These findings contribute to the development of genetic resources for common rust resistance in maize breeding programs.
由[病原菌名称未给出]引起的玉米普通锈病是玉米主要的叶部病害，会导致品质下降和产量损失。为剖析玉米对普通锈病抗性的遗传结构，本研究利用感病温带自交系掖107作为父本，与三个抗病热带玉米自交系（CML312、D39和Y32）杂交，构建了627个F重组自交系（RIL），旨在鉴定玉米普通锈病抗病位点和候选基因。表型数据显示抗性和感病性之间有良好的分离，不同亚群体中观察到不同程度的抗性。观察到显著的基因型效应和基因型×环境互作，遗传力范围为85.7%至92.2%。在三种环境下进行的连锁分析和全基因组关联分析共鉴定出20个QTL和62个显著的SNP。其中，7个主要QTL解释了66%的表型变异。与在不同环境中反复鉴定出的6个SNP进行比较，发现[相关基因未给出]与[相关基因未给出]、[相关基因未给出]和[相关基因未给出]之间存在重叠。单倍型分析表明，这两个SNP的普通锈病抗性均有两种不同的单倍型。基于连锁不平衡衰减图，在这两个SNP上下游20 kb范围内鉴定出三个共定位的候选基因，分别为[基因名称未给出]、[基因名称未给出]和[基因名称未给出]。[基因名称未给出]调控激素调节，[基因名称未给出]控制气孔开闭、与毛状体有关，[基因名称未给出]与植物病害免疫反应相关。此外，我们对在不同环境中反复检测到的另外5个SNP进行了候选基因筛选，鉴定出5个候选基因。这些发现有助于玉米育种计划中普通锈病抗性遗传资源的开发。
