Mendoza-Cerezo Laura, Rodríguez-Rego Jesús M, Macías-García Antonio, Callejas-Marín Antuca, Sánchez-Guardado Luís, Marcos-Romero Alfonso C
Department of Graphic Expression, School of Industrial Engineering, University of Extremadura, Avenida de Elvas, s/n, 06006 Badajoz, Spain.
Department of Mechanical, Energy and Materials Engineering, School of Industrial Engineering, University of Extremadura, Avenida de Elvas, s/n, 06006 Badajoz, Spain.
Polymers (Basel). 2024 May 19;16(10):1437. doi: 10.3390/polym16101437.
Three-dimensional extrusion bioprinting technology aims to become a fundamental tool for tissue regeneration using cell-loaded hydrogels. These biomaterials must have highly specific mechanical and biological properties that allow them to generate biosimilar structures by successive layering of material while maintaining cell viability. The rheological properties of hydrogels used as bioinks are critical to their printability. Correct printability of hydrogels allows the replication of biomimetic structures, which are of great use in medicine, tissue engineering and other fields of study that require the three-dimensional replication of different tissues. When bioprinting cell-loaded hydrogels, a small amount of culture medium can be added to ensure adequate survival, which can modify the rheological properties of the hydrogels. GelMA is a hydrogel used in bioprinting, with very interesting properties and rheological parameters that have been studied and defined for its basic formulation. However, the changes that occur in its rheological parameters and therefore in its printability, when it is mixed with the culture medium necessary to house the cells inside, are unknown. Therefore, in this work, a comparative study of GelMA 100% and GelMA in the proportions 3:1 (GelMA 75%) and 1:1 (GelMA 50%) with culture medium was carried out to determine the printability of the gel (using a device of our own invention), its main rheological parameters and its toxicity after the addition of the medium and to observe whether significant differences in cell viability occur. This raises the possibility of its use in regenerative medicine using a 3D extrusion bioprinter.
三维挤出生物打印技术旨在成为使用负载细胞水凝胶进行组织再生的基础工具。这些生物材料必须具有高度特定的机械和生物学特性，使其能够通过材料的连续分层生成生物相似结构，同时保持细胞活力。用作生物墨水的水凝胶的流变学特性对其可打印性至关重要。水凝胶的正确可打印性允许仿生结构的复制，这在医学、组织工程和其他需要对不同组织进行三维复制的研究领域中非常有用。当生物打印负载细胞的水凝胶时，可以添加少量培养基以确保足够的存活率，这可能会改变水凝胶的流变学特性。GelMA是一种用于生物打印的水凝胶，具有非常有趣的特性和流变学参数，其基本配方已得到研究和定义。然而，当它与容纳细胞所需的培养基混合时，其流变学参数以及因此其可打印性会发生何种变化尚不清楚。因此，在这项工作中，对100%的GelMA以及比例为3:1（GelMA 75%）和1:1（GelMA 50%）的GelMA与培养基进行了比较研究，以确定凝胶的可打印性（使用我们自己发明的设备）、其主要流变学参数以及添加培养基后的毒性，并观察细胞活力是否存在显著差异。这增加了使用3D挤出生物打印机将其用于再生医学的可能性。
