State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China; Center of Stomatology, West China Xiamen Hospital of Sichuan University, Xiamen 361021, Fujian, China.
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China.
Biomater Adv. 2024 Jul;161:213892. doi: 10.1016/j.bioadv.2024.213892. Epub 2024 May 11.
Guided bone regeneration (GBR) stands as an essential modality for craniomaxillofacial bone defect repair, yet challenges like mechanical weakness, inappropriate degradability, limited bioactivity, and intricate manufacturing of GBR membranes hindered the clinical efficacy. Herein, we developed a Janus bacterial cellulose(BC)/MXene membrane through a facile vacuum filtration and etching strategy. This Janus membrane displayed an asymmetric bilayer structure with interfacial compatibility, where the dense layer impeded cell invasion and the porous layer maintained stable space for osteogenesis. Incorporating BC with TiCTx MXene significantly enhanced the mechanical robustness and flexibility of the material, enabling clinical operability and lasting GBR membrane supports. It also contributed to a suitable biodegradation rate, which aligned with the long-term bone repair period. After demonstrating the desirable biocompatibility, barrier role, and osteogenic capability in vitro, the membrane's regenerative potential was also confirmed in a rat cranial defect model. The excellent bone repair performance could be attributed to the osteogenic capability of MXene nanosheets, the morphological cues of the porous layer, as well as the long-lasting, stable regeneration space provided by the GBR membrane. Thus, our work presented a facile, robust, long-lasting, and biodegradable BC/MXene GBR membrane, offering a practical solution to craniomaxillofacial bone defect repair.
引导骨再生（GBR）是颅颌面骨缺损修复的重要方式，但 GBR 膜在机械强度、降解性、生物活性和复杂制造方面存在的挑战，限制了其临床效果。本研究通过简单的真空过滤和蚀刻策略，开发了一种双层细菌纤维素（BC）/MXene 膜。这种双层膜具有界面相容性的不对称双层结构，其中致密层阻止细胞侵入，多孔层保持稳定的成骨空间。BC 与 TiCTx MXene 的结合显著提高了材料的机械强度和柔韧性，使其具有临床可操作性和持久的 GBR 膜支撑能力。同时，它还具有合适的降解率，与长期的骨修复周期相匹配。在体外证明了良好的生物相容性、屏障作用和成骨能力后，该膜在大鼠颅顶骨缺损模型中也证实了其再生潜力。优异的骨修复性能归因于 MXene 纳米片的成骨能力、多孔层的形态线索以及 GBR 膜提供的持久、稳定的再生空间。因此，我们的工作提出了一种简便、坚固、持久、可生物降解的 BC/MXene GBR 膜，为颅颌面骨缺损修复提供了一种实用的解决方案。
