North M L, Lecocq J, Roth-Fritsch M, Piffaut M C, Asch L
Ann Biol Clin (Paris). 1985;43(6):861-6.
The object of the present study was to define the optimal conditions for the quantitative determination of fibronectin in synovial fluid by laser nephelometry and to determine the diagnostic importance of this analysis in rheumatology. The results show that this technique is sensitive (threshold sensitivity is 70 mg/l if the sample is diluted 1/30 and 5 mg/l for a sample diluted to 1/2), accurate, reproducible (coefficient of variation less than 10%), specific, simple and rapid. The response is linear for dilutions from 1/30 to 1/240. Fibronectin P and fibronectin S were determined in 104 subjects who had contracted an arthropathy; 26 cases of rheumatoid polyarthritis, 43 mechanical arthropathies and 35 cases of non-rheumatoid arthritis. the plasma fibronectin does not vary significantly from one group of arthropathies to another but the mean value for the total population of arthropathies is significantly lower than that for a control group. In the synovial fluid the mean value for fibronectin in the patients with rheumatoid arthritis (583 + 76 mg/l) is very considerably higher than that in non-rheumatoid (379 + 58 mg/l) or mechanical (367 + 32 mg/l) arthritis patients. Thus, the determination of synovial fibronectin may provide useful information for the etiological diagnosis of inflammatory arthropathies.
本研究的目的是确定用激光散射比浊法定量测定滑液中纤连蛋白的最佳条件，并确定该分析在风湿病学中的诊断重要性。结果表明，该技术灵敏（若样品稀释1/30，阈值灵敏度为70mg/l；若样品稀释至1/2，阈值灵敏度为5mg/l）、准确、可重复（变异系数小于10%）、特异、简单且快速。对于1/30至1/240的稀释度，响应呈线性。对104例患有关节病的受试者测定了纤连蛋白P和纤连蛋白S；其中类风湿性多关节炎26例，机械性关节病43例，非类风湿性关节炎35例。不同组的关节病患者血浆纤连蛋白无显著差异，但关节病患者总体的平均值显著低于对照组。在滑液中，类风湿性关节炎患者的纤连蛋白平均值（583 + 76mg/l）显著高于非类风湿性关节炎（379 + 58mg/l）或机械性关节炎（367 + 32mg/l）患者。因此，滑液纤连蛋白的测定可为炎症性关节病的病因诊断提供有用信息。
