Kim Min-Ji, Mangal Utkarsh, Seo Ji-Young, Kim Ji-Yun, Kim Ji-Yeong, Ryu Jeong-Hyun, Kim Hee-Jin, Lee Kee-Joon, Kwon Jae-Sung, Choi Sung-Hwan
Division in Anatomy and Developmental Biology, Department of Oral Biology, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea; Department of Orthodontics, Institute of Craniofacial Deformity, Yonsei University College of Dentistry, Seoul, Republic of Korea; BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
J Dent. 2024 Sep;148:105054. doi: 10.1016/j.jdent.2024.105054. Epub 2024 May 23.
To create bacteria-resistant dental CAD-CAM blocks with a biofilm-resistant effect by incorporating Nano-crystalline ceramic and polymer (NCP) with 2-methacryloyloxyethyl phosphorylcholine (MPC) and sulfobetaine methacrylate (SBMA) and at an equimolar ratio, referred to as MS.
Experimental groups comprised NCP blocks containing zwitterions at 0.15wt% (MS015) and 0.45wt% (MS045). NCP blocks without MS served as control (CTRL). Flexural strength, surface hardness, water sorption and solubility, photometric properties, and cytotoxicity were assessed for all samples. Additionally, the resistance to single and multi-species bacterial adhesion was investigated.
MS045 showed significant reduction in flexural strength (P < 0.01) compared to both CTRL and MS015. Both MS015 and MS045 showed significantly increased water sorption and significant reduction in water solubility compared to CTRL. Light transmission remained consistent across all MS content levels, but the irradiance value decreased by 12 % in the MS045 group compared to the MS015 group. Notably, compared to the CTRL group, the MS015 group exhibited enhanced resistance to adhesion by Porphyromonas gingivalis and a multi-species salivary biofilm, with biofilm thickness and biomass reduced by 45 % and 56 %, respectively.
NCP containing 0.15 % MS can effectively reduce adhesion of multiple species of bacteria while maintaining physical and mechanical properties.
NCP integrating zwitterions is clinically advantageous in resisting bacterial adhesion at internal and external margins of milled indirect restoration.
通过将纳米晶陶瓷与聚合物（NCP）与2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）和甲基丙烯酰基磺基甜菜碱（SBMA）以等摩尔比混合（称为MS），制备具有抗生物膜作用的抗菌牙科CAD-CAM块体。
实验组包括含有0.15wt%（MS015）和0.45wt%（MS045）两性离子的NCP块体。不含MS的NCP块体作为对照（CTRL）。评估所有样品的弯曲强度、表面硬度、吸水率和溶解度、光度特性及细胞毒性。此外，还研究了对单一和多种细菌粘附的抗性。
与CTRL和MS015相比，MS045的弯曲强度显著降低（P < 0.01）。与CTRL相比，MS015和MS045的吸水率均显著增加，溶解度显著降低。所有MS含量水平的透光率保持一致，但MS045组的辐照度值比MS015组降低了12%。值得注意的是，与CTRL组相比，MS015组对牙龈卟啉单胞菌和多种唾液生物膜的粘附抗性增强，生物膜厚度和生物量分别减少了45%和56%。
含有0.15%MS的NCP能有效降低多种细菌的粘附，同时保持物理和机械性能。
整合两性离子的NCP在抵抗铣削间接修复体内外边缘细菌粘附方面具有临床优势。
