Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, Korea.
BK21 FOUR Project, Yonsei University College of Dentistry, Seoul 03722, Korea.
Int J Mol Sci. 2021 Jan 3;22(1):417. doi: 10.3390/ijms22010417.
Poly(methyl methacralyate) (PMMA) has long been used in dentistry as a base polymer for dentures, and it is recently being used for the 3D printing of dental materials. Despite its many advantages, its susceptibility to microbial colonization remains to be overcome. In this study, the interface between 3D-printed PMMA specimens and oral salivary biofilm was studied following the addition of zwitterionic materials, 2-methacryloyloxyethyl phosphorylcholine (MPC) or sulfobetaine methacrylate (SB). A significant reduction in bacterial and biofilm adhesions was observed following the addition of MPC or SB, owing to their protein-repellent properties, and there were no significant differences between the two test materials. Although the mechanical properties of the tested materials were degraded, the statistical value of the reduction was minimal and all the properties fulfilled the requirements set by the International Standard, ISO 20795-2. Additionally, both the test materials maintained their resistance to biofilm when subjected to hydrothermal fatigue, with no further deterioration of the mechanical properties. Thus, novel 3D-printable PMMA incorporated with MPC or SB shows durable oral salivary biofilm resistance with maintenance of the physical and mechanical properties.
聚甲基丙烯酸甲酯（PMMA）长期以来一直被用作义齿的基础聚合物，并且最近还被用于牙科材料的 3D 打印。尽管它有许多优点，但它对微生物定植的易感性仍有待克服。在这项研究中，在添加两性离子材料 2-（甲基丙烯酰氧）乙基磷酸胆碱（MPC）或磺基甜菜碱甲基丙烯酸酯（SB）后，研究了 3D 打印 PMMA 标本与口腔唾液生物膜之间的界面。由于其蛋白质排斥特性，添加 MPC 或 SB 后观察到细菌和生物膜黏附的显著减少，并且两种测试材料之间没有显着差异。尽管测试材料的机械性能降低，但减少的统计值很小，所有性能都满足国际标准 ISO 20795-2 规定的要求。此外，当经受湿热疲劳时，两种测试材料均保持对生物膜的抵抗力，而机械性能没有进一步恶化。因此，新型 3D 可打印 PMMA 与 MPC 或 SB 结合具有耐用的口腔唾液生物膜抵抗力，同时保持物理和机械性能。
