Rosdahl V T
Acta Pathol Microbiol Immunol Scand B. 1985 Dec;93(6):383-8. doi: 10.1111/j.1699-0463.1985.tb02906.x.
Localisation of the penicillinase gene was studied in a total of 104 inducible Staphylococcus aureus strains selected from 10,229 bacteraemia strains isolated in the years 1957-1981. The strains represented all possible combinations of the most common patterns of phage type and susceptibility to heavy metals (cadmium, arsenate and mercury) and antibiotics. The material was further supplemented with epidemically occurring strains. Localisation of the penicillinase gene was established by isolation of penicillinase-negative variants and analysis of DNA-bands on CsCl gradients and gel-electrophoresis. Fifty-five strains contained a penicillinase plasmid; 49 had a chromosomal location. The genetic location was mainly connected with phage types. All strains belonging to the 83A complex and the majority of the strains of the 52, 52A, 80, 81 complex and of phage type 95 contained a penicillinase plasmid, whereas chromosomal location was found in all strains of group II and of the 94,96 complex. In all strains but three (group III) the resistances to heavy metals were located on the penicillinase plasmid. Association between the penicillinase plasmid and genes coding for resistance to other antibiotics was found in one tetracycline resistant strain only. Three micro-constitutive strains had chromosomally located penicillinase gene, whereas one macro-constitutive strain contained a penicillinase plasmid.
对从1957年至1981年间分离出的10229株菌血症菌株中挑选出的104株诱导性金黄色葡萄球菌菌株进行了青霉素酶基因定位研究。这些菌株代表了噬菌体类型以及对重金属（镉、砷酸盐和汞）和抗生素敏感性的最常见模式的所有可能组合。该材料还补充了流行菌株。通过分离青霉素酶阴性变体以及分析氯化铯梯度和凝胶电泳上的DNA条带，确定了青霉素酶基因的定位。55株菌株含有青霉素酶质粒；49株的青霉素酶基因位于染色体上。基因定位主要与噬菌体类型有关。所有属于83A复合体的菌株以及52、52A、80、81复合体和噬菌体类型95的大多数菌株都含有青霉素酶质粒，而在II组和94、96复合体的所有菌株中发现青霉素酶基因位于染色体上。除三株菌株（III组）外，所有菌株对重金属的抗性都位于青霉素酶质粒上。仅在一株耐四环素菌株中发现了青霉素酶质粒与编码对其他抗生素抗性的基因之间的关联。三株微组成型菌株的青霉素酶基因位于染色体上，而一株宏组成型菌株含有青霉素酶质粒。
