Melia Sri, Juliyarsi Indri, Kurnia Yulianti Fitri, Aritonang Salam N, Rusdimansyah Rusdimansyah, Sukma Ade, Setiawan Rizki Dwi, Pratama Yudha Endra, Supandil Doni
Department of Animal Products Technology, Faculty of Animal Science, Universitas Andalas, Padang, 25163, Indonesia.
Department of Animal Production, Faculty of Animal Science, Universitas Andalas, Padang, 25163, Indonesia.
Vet World. 2024 Apr;17(4):785-795. doi: 10.14202/vetworld.2024.785-795. Epub 2024 Apr 10.
Stingless bees are generally found in tropical countries, including Indonesia. In West Sumatra, stingless bees are known as , consist of several species with different characteristics; however, the properties of honey produced by stingless bees have not yet been explored. This study aimed to determine the physicochemical, antioxidant, and antimicrobial activities as well as the microbiota profile of stingless bee honey from the bee species , , , and that are intensively developed in West Sumatra, Indonesia.
Honey produced by the stingless bee species , . , , and originating in West Sumatra was examined in the present study. The physicochemical properties (Association of Official Analytical Chemists), antioxidant activity (2,2-diphenyl-1-picrylhydrazyl technique), total phenols (Folin-Ciocalteu method), antimicrobial activity (Agar-Well diffusion test), total lactic acid bacteria, and microbiota diversity were measured in stingless bee honey samples.
Stingless bee species significantly affected the physicochemical properties, antioxidant activity, total phenolic content, antimicrobial activity, and total lactic acid bacteria (p = 0.05), except for the crude fiber content. The carbohydrate profiles of honey produced by and were dominated by monosaccharides, whereas those of honey from and were dominated by disaccharides. In terms of antioxidant activity (half maximal inhibitory concentration [IC] value), there were no significant differences (p > 0.05) between honey from , , and , but there were significant differences (p > 0.05) between honey from . The honey of and had the highest total phenolic content (65.65 ± 14.00 and 69.78 ± 8.06, respectively). In addition, honey from the four stingless bee species showed antimicrobial activity against the pathogenic bacteria , , , and . From the principal co-ordinate analysis (PCoA) results, it can be concluded that the microbiota profiles of the four stingless bee honey samples differed.
The results showed that honey from , , , and has different physicochemical characteristics, antioxidant activity, antimicrobial activity, and microbiota diversity. By knowing the content of this stingless bee honey, the results of this study can be used as information that this stingless bee honey has the potential as a functional food that is beneficial for health.
无刺蜂通常见于包括印度尼西亚在内的热带国家。在西苏门答腊，无刺蜂被称为 ，由几种具有不同特征的物种组成；然而，无刺蜂所产蜂蜜的特性尚未得到探索。本研究旨在测定印度尼西亚西苏门答腊大量养殖的 、 、 及 这几种无刺蜂所产蜂蜜的理化性质、抗氧化活性、抗菌活性以及微生物群谱。
本研究检测了原产于西苏门答腊的 、 、 及 这几种无刺蜂所产的蜂蜜。测定了无刺蜂蜂蜜样品的理化性质（官方分析化学师协会方法）、抗氧化活性（2,2-二苯基-1-苦基肼法）、总酚含量（福林-西奥尔特法）、抗菌活性（琼脂扩散法）、总乳酸菌数量及微生物群多样性。
无刺蜂物种对理化性质、抗氧化活性、总酚含量、抗菌活性及总乳酸菌数量有显著影响（p = 0.05），粗纤维含量除外。 和 所产蜂蜜的碳水化合物谱以单糖为主，而 和 所产蜂蜜的碳水化合物谱以二糖为主。在抗氧化活性方面（半数最大抑制浓度[IC]值）， 、 及 所产蜂蜜之间无显著差异（p > 0.05），但 所产蜂蜜之间存在显著差异（p > 0.05）。 和 所产蜂蜜的总酚含量最高（分别为65.65 ± 14.00和69.78 ± 8.06）。此外，这四种无刺蜂所产蜂蜜对病原菌 、 、 及 均表现出抗菌活性。从主坐标分析（PCoA）结果可以得出，这四种无刺蜂蜂蜜样品的微生物群谱不同。
结果表明， 、 、 及 所产蜂蜜具有不同的理化特性、抗氧化活性、抗菌活性及微生物群多样性。通过了解这种无刺蜂蜂蜜的成分，本研究结果可作为信息表明这种无刺蜂蜂蜜具有作为有益健康的功能性食品的潜力。
