Ng Wen-Jie, Sit Nam-Weng, Ooi Peter Aun-Chuan, Ee Kah-Yaw, Lim Tuck-Meng
Faculty of Science, Universiti Tunku Abdul Rahman, Kampar 31900, Malaysia.
Antibiotics (Basel). 2020 Dec 5;9(12):871. doi: 10.3390/antibiotics9120871.
Scientific studies about the antibacterial effects of honeydew honey produced by the stingless bee are very limited. In this study, the antibacterial activities of 46 blossom and honeydew honeys produced by both honey bees and stingless bees were evaluated and compared. All bacterial isolates showed varying degrees of susceptibility to blossom and honeydew honeys produced by the honey bee () and stingless bee ( and ) in agar-well diffusion. All stingless bee honeys managed to inhibit all the isolates but only four out of 23 honey bee honeys achieved that. In comparison with , was found to be more susceptible to the antibacterial effects of honey. Bactericidal effects of stingless bee honeys on were determined with the measurement of endotoxins released due to cell lysis. Based on the outcomes, the greatest antibacterial effects were observed in honeydew honey produced by . Scanning electron microscopic images revealed the morphological alteration and destruction of due to the action of this honey. The combination of this honey with antibiotics showed synergistic inhibitory effects on clinical isolates. This study revealed that honeydew honey produced by stingless bee has promising antibacterial activity against pathogenic bacteria, including antibiotic resistant strains.
关于无刺蜂所产甘露蜜抗菌作用的科学研究非常有限。在本研究中，对蜜蜂和无刺蜂所产的46种花蜜和甘露蜜的抗菌活性进行了评估和比较。在琼脂孔扩散实验中，所有细菌分离株对蜜蜂（）和无刺蜂（和）所产的花蜜和甘露蜜均表现出不同程度的敏感性。所有无刺蜂蜂蜜都能抑制所有分离株，但23种蜜蜂蜂蜜中只有4种能做到这一点。与相比，发现对蜂蜜的抗菌作用更敏感。通过测量细胞裂解释放的内毒素来确定无刺蜂蜂蜜对的杀菌作用。基于这些结果，在无刺蜂所产的甘露蜜中观察到了最大的抗菌作用。扫描电子显微镜图像显示，由于这种蜂蜜的作用，出现了形态改变和破坏。这种蜂蜜与抗生素的组合对临床分离株显示出协同抑制作用。本研究表明，无刺蜂所产的甘露蜜对包括抗生素耐药菌株在内的病原菌具有有前景的抗菌活性。
