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眼部组织提取与匀浆标准化方案

A Standardized Protocol for Extraction and Homogenization of Ocular Tissues.

作者信息

Hammid Anam

机构信息

School of Pharmacy, University of Eastern Finland, Yliopistonrinne 3, Kuopio, Finland.

出版信息

Bio Protoc. 2024 May 20;14(10):e4988. doi: 10.21769/BioProtoc.4988.

DOI:10.21769/BioProtoc.4988
PMID:38798978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11116895/
Abstract

The eye is a complex organ composed of multiple tissues in anterior and posterior eye segments. Malfunctions of any of these tissues can lead to ocular diseases and loss of vision. A detailed understanding of the ocular anatomy and physiology in animal models and humans contributes to the development of ocular drugs by enabling studies on drug delivery and clearance routes, pharmacokinetics, and toxicity. This protocol provides step-by-step instructions for the extraction and homogenization of ocular tissues for enzymatic and proteomics analyses. Key features • Suitable protocol for the extraction and isolation of ocular tissue from humans and laboratory animals (rabbit, pig, rat, mouse) while minimizing cross-contamination. • Hard or soft tissue homogenates can be prepared efficiently using a Bead Ruptor homogenizer. • Allows to determine the protein contents in prepared homogenates.

摘要

眼睛是一个复杂的器官，由眼前段和眼后段的多种组织组成。这些组织中任何一个出现故障都可能导致眼部疾病和视力丧失。通过对动物模型和人类的眼部解剖学和生理学进行详细了解，能够开展关于药物递送和清除途径、药代动力学及毒性的研究，从而有助于眼部药物的开发。本方案提供了用于眼部组织提取和匀浆以进行酶学和蛋白质组学分析的分步说明。关键特性 • 适用于从人类和实验动物（兔子、猪、大鼠、小鼠）提取和分离眼部组织，同时将交叉污染降至最低。 • 使用珠磨均质器可高效制备硬组织或软组织匀浆。 • 能够测定所制备匀浆中的蛋白质含量。

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本文引用的文献

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Carboxylesterase Activities and Protein Expression in Rabbit and Pig Ocular Tissues.兔和猪眼组织中的羧酸酯酶活性及蛋白表达
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30 多年来盲症和远距离及近距离视力损伤流行率的变化趋势：全球疾病负担研究的分析。
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