Department of Ultrasound Medicine, Tangdu Hospital, Air Force Medical University, Xi'an, People's Republic of China.
Department of Pharmaceutical Chemistry and Analysis, School of Pharmacy, Air Force Medical University, Xi'an, People's Republic of China.
Int J Nanomedicine. 2024 May 22;19:4651-4665. doi: 10.2147/IJN.S462917. eCollection 2024.
Recently, nanobubbles (NBs) have gained significant traction in the field of tumor diagnosis and treatment owing to their distinctive advantages. However, the application of NBs is limited due to their restricted size and singular reflection section, resulting in low ultrasonic reflection.
We synthesized a nano-scale ultrasound contrast agent (IR783-SiONPs@NB) by encapsulating SiO nanoparticles in an IR783-labeled lipid shell using an improved film hydration method. We characterized its physicochemical properties, examined its microscopic morphology, evaluated its stability and cytotoxicity, and assessed its contrast-enhanced ultrasound imaging capability both in vitro and in vivo.
The results show that IR783-SiONPs@NB had a "donut-type" composite microstructure, exhibited uniform particle size distribution (637.2 ± 86.4 nm), demonstrated excellent stability (30 min), high biocompatibility, remarkable tumor specific binding efficiency (99.78%), and an exceptional contrast-enhanced ultrasound imaging capability.
Our newly developed multiple scattering NBs with tumor targeting capacity have excellent contrast-enhanced imaging capability, and they show relatively long contrast enhancement duration in solid tumors, thus providing a new approach to the structural design of NBs.
由于其独特的优势，纳米气泡（NBs）在肿瘤诊断和治疗领域得到了广泛关注。然而，由于其尺寸受限和单一反射截面，导致超声反射率低，限制了 NBs 的应用。
我们通过改进的薄膜水化法，将硅纳米颗粒包裹在 IR783 标记的脂质壳中，合成了一种纳米级超声对比剂（IR783-SiONPs@NB）。我们对其理化性质进行了表征，考察了其微观形态，评估了其稳定性和细胞毒性，并评估了其在体外和体内的对比增强超声成像能力。
结果表明，IR783-SiONPs@NB 具有“甜甜圈型”复合微观结构，粒径分布均匀（637.2±86.4nm），稳定性好（30min），生物相容性高，具有优异的肿瘤特异性结合效率（99.78%）和出色的对比增强超声成像能力。
我们新开发的具有肿瘤靶向能力的多散射 NBs 具有优异的对比增强成像能力，在实体瘤中具有相对较长的对比增强持续时间，为 NBs 的结构设计提供了一种新方法。
