Lott T J, Yang J H, Ye J H, Wallace D C
Department of Biology, Emory University, Atlanta, GA 30322.
Comput Appl Biosci. 1985 Dec;1(4):249-52. doi: 10.1093/bioinformatics/1.4.249.
We have developed an inexpensive yet versatile microcomputer-based system for quantitating light intensity levels in autoradiographs. This system employs a standard video camera interfaced to an analog-to-digital convertor. A program has been written for this system which can measure intensities within a defined region of an autoradiograph, permitting an easy and accurate quantitation of spots or bands of irregular shape.
