Mittermeier Fabian, Fischer Fabienne, Hauke Sebastian, Hirschmann Peter, Weuster-Botz Dirk
Chair of Biochemical Engineering, Technical University of Munich, 85748 Garching, Germany.
Bavarian Milling Confederation (Bayerischer Müllerbund e.V.), 80333 Munich, Germany.
BioTech (Basel). 2024 May 18;13(2):15. doi: 10.3390/biotech13020015.
The enzymatic hydrolysis of agricultural residues like wheat bran enables the valorization of otherwise unused carbon sources for biotechnological processes. The co-culture of and with wheat bran particles as substrate produces an enzyme set consisting of xylanases, amylases, and cellulases that is suitable to degrade lignocellulosic biomass to sugar monomers (D-glucose, D-xylose, and L-arabinose). An integrated one-pot process for enzyme production followed by hydrolysis in stirred tank bioreactors resulted in hydrolysates with overall sugar concentrations of 32.3 g L and 24.4 g L at a 25 L and a 1000 L scale, respectively, within 86 h. Furthermore, the residual solid biomass consisting of fermented wheat bran with protein-rich fungal mycelium displays improved nutritional properties for usage as animal feed due to its increased content of sugars, protein, and fat.
对麦麸等农业残余物进行酶促水解，可实现对生物技术过程中原本未被利用的碳源的增值利用。以麦麸颗粒为底物，将[具体两种微生物，原文未给出]与[另一种微生物，原文未给出]共培养，可产生一组由木聚糖酶、淀粉酶和纤维素酶组成的酶，该酶组适合将木质纤维素生物质降解为糖单体（D-葡萄糖、D-木糖和L-阿拉伯糖）。在搅拌罐式生物反应器中，先进行酶生产，然后进行水解的一体化一锅法工艺，在25升规模和1000升规模下，分别在86小时内得到了总糖浓度为32.3克/升和24.4克/升的水解产物。此外，由富含蛋白质的真菌菌丝体的发酵麦麸组成的残余固体生物质，由于其糖、蛋白质和脂肪含量增加，作为动物饲料使用时显示出改善的营养特性。
