Uprety Laxmi Prasad, Lee Chang-Gun, Oh Kang-Il, Jeong Hyesoo, Yeo Subin, Yong Yoonjoong, Seong Je Kyung, Kim Il Yong, Go Hyesun, Park Eunkuk, Jeong Seon-Yong
Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, South Korea; Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 16499, South Korea.
Department of Biomedical Laboratory Science, College of Software and Digital Healthcare Convergence, Yonsei University MIRAE Campus, Wonju 26493, South Korea.
Biomed Pharmacother. 2024 Jul;176:116799. doi: 10.1016/j.biopha.2024.116799. Epub 2024 May 27.
The overstoring of surplus calories in mature adipocytes causes obesity and abnormal metabolic activity. The anti-obesity effect of a Celosia cristata (CC) total flower extract was assessed in vitro, using 3T3-L1 pre-adipocytes and mouse adipose-derived stem cells (ADSCs), and in vivo, using high-fat diet (HFD)-treated C57BL/6 male mice.
CC extract was co-incubated during adipogenesis in both 3T3-L1 cells and ADSCs. After differentiation, lipid droplets were assessed by oil red O staining, adipogenesis and lipolytic factors were evaluated, and intracellular triglyceride and glycerol concentrations were analyzed. For in vivo experiments, histomorphological analysis, mRNA expression levels of adipogenic and lipolytic factors in adipose tissue, blood plasma analysis, metabolic profiles were investigated.
CC treatment significantly prevented adipocyte differentiation and lipid droplet accumulation, reducing adipogenesis-related factors and increasing lipolysis-related factors. Consequently, the intracellular triacylglycerol content was diminished, whereas the glycerol concentration in the cell supernatant increased. Mice fed an HFD supplemented with the CC extract exhibited decreased HFD-induced weight gain with metabolic abnormalities such as intrahepatic lipid accumulation and adipocyte hypertrophy. Improved glucose utilization and insulin sensitivity were observed, accompanied by the amelioration of metabolic disturbances, including alterations in liver enzymes and lipid profiles, in CC-treated mice. Moreover, the CC extract helped restore the disrupted energy metabolism induced by the HFD, based on a metabolic animal monitoring system.
This study suggests that CC total flower extract is a potential natural herbal supplement for the prevention and management of obesity.
成熟脂肪细胞中多余热量的过度储存会导致肥胖和异常代谢活动。使用3T3-L1前脂肪细胞和小鼠脂肪来源干细胞（ADSCs）在体外评估了鸡冠花（CC）总花提取物的抗肥胖作用，并使用高脂饮食（HFD）处理的C57BL/6雄性小鼠在体内进行了评估。
在3T3-L1细胞和ADSCs的脂肪生成过程中共同孵育CC提取物。分化后，通过油红O染色评估脂滴，评估脂肪生成和脂解因子，并分析细胞内甘油三酯和甘油浓度。对于体内实验，进行了组织形态学分析、脂肪组织中脂肪生成和脂解因子的mRNA表达水平、血浆分析、代谢谱研究。
CC处理显著阻止了脂肪细胞分化和脂滴积累，减少了脂肪生成相关因子并增加了脂解相关因子。因此，细胞内三酰甘油含量减少，而细胞上清液中的甘油浓度增加。喂食添加了CC提取物的HFD的小鼠表现出HFD诱导的体重增加减少，伴有肝内脂质积累和脂肪细胞肥大等代谢异常。在CC处理的小鼠中观察到葡萄糖利用率和胰岛素敏感性提高，同时代谢紊乱得到改善，包括肝酶和血脂谱的改变。此外，基于代谢动物监测系统，CC提取物有助于恢复由HFD诱导的能量代谢紊乱。
本研究表明，CC总花提取物是一种潜在的用于预防和管理肥胖的天然草药补充剂。
