Lian Yingzhu, Fu Guiming, Liang Xinmei, He Xinchao, Xu Jin, Fan Haowei, Wan Yin
State Key Laboratory of Food Science and Resources, College of Food Science & Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
State Key Laboratory of Food Science and Resources, College of Food Science & Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Int J Biol Macromol. 2024 Jun;271(Pt 2):132687. doi: 10.1016/j.ijbiomac.2024.132687. Epub 2024 May 26.
Caffeioyl quinic acids and polysaccharides from Artemisia selengensis Turcz are considered potential bioactive substances for hyperuricemia (HUA) treatment. While the mechanism of multi-component combined intervention of polysaccharides and dicaffeoylquinic acids (diCQAs) is not yet clear. In this study, we investigated the effect of A. selengensis Turcz leaves polysaccharides (APS) on the HUA treatment with diCQAs in vitro by direct inhibition of XOD activities and in vivo by using animal model. The results showed that APS had almost no inhibitory effect on XOD activities in vitro, but the inhibitory activity of diCQAs on XOD was affected by changes in inhibition type and inhibition constant. Compared to APS and diCQAs alone, high-dose APS and diCQAs in combination (ADPSh) could significantly reduce the production of uric acid (16.38 % reduction compared to diCQAs group) and oxidative stress damage. Additionally, this combined therapy showed promise in restoring the gut microbiota balance and increasing the short-chain fatty acids levels. The results suggested that APS and diCQAs in combination could be a potential inhibitor for HUA treatment.
藜蒿中的咖啡酰奎宁酸和多糖被认为是治疗高尿酸血症（HUA）的潜在生物活性物质。然而，多糖和二咖啡酰奎宁酸（diCQAs）多组分联合干预的机制尚不清楚。在本研究中，我们通过直接抑制XOD活性在体外研究了藜蒿叶多糖（APS）对diCQAs治疗HUA的影响，并通过动物模型在体内进行了研究。结果表明，APS在体外对XOD活性几乎没有抑制作用，但diCQAs对XOD的抑制活性受抑制类型和抑制常数变化的影响。与单独使用APS和diCQAs相比，高剂量APS和diCQAs联合使用（ADPSh）可显著降低尿酸生成（与diCQAs组相比降低16.38%）和氧化应激损伤。此外，这种联合疗法在恢复肠道微生物群平衡和提高短链脂肪酸水平方面显示出前景。结果表明，APS和diCQAs联合使用可能是治疗HUA的潜在抑制剂。
