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不同O亚群的大肠杆菌O128菌株的肠毒素产生、定居因子抗原I的存在以及对HeLa细胞的黏附情况。

Enterotoxin production, presence of colonization factor antigen I, and adherence to HeLa cells by Escherichia coli O128 strains belonging to different O subgroups.

作者信息

Guth B E, Silva L M, Scaletsky I C, Toledo M R, Trabulsi L R

出版信息

Infect Immun. 1985 Jan;47(1):338-40. doi: 10.1128/iai.47.1.338-340.1985.

Abstract

Strains of three subgroups of Escherichia coli O128 were studied. Enterotoxin production was observed in 30 (91%) O128ac strains, whereas strains of subgroups O128ab and O128ad were not toxigenic. CFA/I was only found in two serotypes of subgroup O128ac, all of them producing heat-stable enterotoxin except for one which produced both toxins. None of the strains studied produced CFA/II. In a binding test with HeLa cells, localized adherence was found only in strains of subgroup O128ab; diffuse adherence occurred in strains of subgroup O128ac. As flagellar antigens were specific in subgroups ab and ac and toxin production was observed only in subgroup ac, the present results suggest that subgroup and serotype are useful markers for O128 strains that are enterotoxigenic or enteropathogenic.

摘要

对大肠杆菌O128三个亚组的菌株进行了研究。在30株(91%)O128ac菌株中观察到肠毒素产生,而O128ab和O128ad亚组的菌株不产毒。CFA/I仅在O128ac亚组的两种血清型中发现,除一株产生两种毒素外,其余均产生热稳定肠毒素。所研究的菌株均未产生CFA/II。在与HeLa细胞的结合试验中,仅在O128ab亚组的菌株中发现局部黏附;O128ac亚组的菌株出现弥漫性黏附。由于ab和ac亚组的鞭毛抗原具有特异性,且仅在ac亚组中观察到毒素产生,目前的结果表明,亚组和血清型是产肠毒素或致肠病的O128菌株的有用标志物。

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