Guth B E, Trabulsi L R
J Clin Microbiol. 1985 Oct;22(4):626-8. doi: 10.1128/jcm.22.4.626-628.1985.
The usefulness of antisera in detecting enterotoxigenic Escherichia coli (ETEC) strains in Sao Paulo was evaluated. Polyvalent antisera detected 49% of ETEC isolates and were more effective in identifying E. coli that produced heat-labile and heat-stable enterotoxins and in strains that produced only heat-stable enterotoxin. ETEC strains not detected by the antisera belonged to different serogroups not isolated in Sao Paulo before; 34% of these strains had undetermined O antigens, and most of them produced only heat-labile toxin. A variation of serogroups over time was especially observed among strains that produced heat-stable toxin. The importance of H-antigen determinations in the effectiveness of ETEC diagnosis by serological methods became evident, as non-ETEC strains were also detected by polyvalent antisera, but their serotypes were different from those of ETEC strains. Although antisera can be used to identify O:H types of ETEC strains with accuracy, serotyping cannot be recommended for routine diagnosis. However, such a procedure may be useful for studying outbreaks of ETEC diarrhea if the involved serotypes are already known.
对圣保罗市用于检测产肠毒素大肠杆菌（ETEC）菌株的抗血清的效用进行了评估。多价抗血清可检测出49%的ETEC分离株，在鉴定产生不耐热和耐热肠毒素的大肠杆菌以及仅产生耐热肠毒素的菌株方面更有效。未被抗血清检测出的ETEC菌株属于圣保罗市以前未分离到的不同血清群；这些菌株中有34%的O抗原未确定，且大多数仅产生不耐热毒素。特别是在产生耐热毒素的菌株中观察到血清群随时间的变化。H抗原测定在ETEC血清学诊断有效性中的重要性变得明显，因为多价抗血清也能检测出非ETEC菌株，但其血清型与ETEC菌株不同。尽管抗血清可准确用于鉴定ETEC菌株的O:H型，但血清分型不推荐用于常规诊断。然而，如果已知相关血清型，这样的程序可能有助于研究ETEC腹泻的暴发。
