评价用于检测血清抗副球孢子菌抗体的商业免疫扩散试剂。
Evaluation of commercial immunodiffusion reagents for detecting serum anti-Paracoccidioides antibodies.
机构信息
Universidade Federal do Paraná, Hospital de Clínicas, Curitiba, PR, Brasil.
Center of Expertise in Mycology Radboudumc/CWZ. Nijmegen, the Netherlands.
出版信息
Rev Soc Bras Med Trop. 2024 May 27;57:e007052024. doi: 10.1590/0037-8682-0094-2024. eCollection 2024.
BACKGROUND
Accurate diagnosis of paracoccidioidomycosis is crucial for improving patient outcomes. Paracoccidioides antibody detection by double immunodiffusion (DID) is a convenient diagnostic tool, but testing performance can vary based on certain factors.
METHODS
We assessed DID performance using a commercially prepared Paracoccidioides reagents (IMMY, USA), involving 40 serum specimens, including 20 from patients with proven paracoccidioidomycosis and 20 from patients without the disease. The DID test demonstrated a sensitivity of 90% (95% CI=68%-99%) and a specificity of 100% (95% CI=83%-100%).
CONCLUSIONS
Our findings suggest that DID using commercial reagents may provide a feasible tool with satisfactory testing performance for anti-Paracoccidioides antibody detection.
背景
准确诊断球孢子菌病对于改善患者预后至关重要。双扩散免疫检测（DID）检测对球孢子菌的抗体是一种便捷的诊断工具，但检测性能可能因某些因素而异。
方法
我们使用商业制备的球孢子菌试剂（IMMY，美国）评估 DID 的性能，涉及 40 份血清标本，包括 20 份来自确诊球孢子菌病患者的标本和 20 份来自无该疾病患者的标本。DID 试验的敏感性为 90%（95%CI=68%-99%），特异性为 100%（95%CI=83%-100%）。
结论
我们的研究结果表明，使用商业试剂的 DID 可能为抗球孢子菌抗体检测提供一种可行的、具有满意检测性能的工具。
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