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Repressor for the sn-glycerol-3-phosphate regulon of Escherichia coli K-12: cloning of the glpR gene and identification of its product.

作者信息

Schweizer H, Boos W, Larson T J

出版信息

J Bacteriol. 1985 Feb;161(2):563-6. doi: 10.1128/jb.161.2.563-566.1985.

DOI:10.1128/jb.161.2.563-566.1985
PMID:3881401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214919/
Abstract

The glpR gene encoding the repressor for the glp regulon of Escherichia coli was cloned from a library of HindIII DNA fragments established in bacteriophage lambda. Phages harboring glpR were isolated by selection for sn-glycerol-3-phosphate dehydrogenase function encoded by glpD, which is adjacent to glpR on the E. coli linkage map. Restriction endonuclease analysis and recloning of DNA fragments localized glpR to a 3-kilobase-pair EcoRI-SalI segment of DNA. Strains exhibiting constitutive expression of the glp operons were strongly repressed after introduction of multicopy plasmids containing the glpR gene. Analysis of proteins labeled in minicells harboring either glpR+ recombinant plasmids or a glpR::Tn5 derivative showed that the glpR gene product is a protein with an apparent molecular weight of 33,000.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee6/214919/2586598d1ff8/jbacter00225-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee6/214919/2586598d1ff8/jbacter00225-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee6/214919/2586598d1ff8/jbacter00225-0101-a.jpg

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain.大肠杆菌K-12的sn-甘油-3-磷酸调节子的阻遏物：一级结构及DNA结合结构域的鉴定
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