Schweizer H, Boos W
Mol Gen Genet. 1983;192(1-2):177-86. doi: 10.1007/BF00327664.
Using a novel positive selection method for G3P transport activity, lambda phages that carry either all or part of ugp, the genes of the pho regulon-dependent G3P transport system of Escherichia coli were isolated from a library of EcoRI fragments of Escherichia coli established in lambda gt7. By subcloning EcoRI fragments carried by the different phages into the multicopy plasmids pACYC184 and pUR222, it was shown that two chromosomal fragments of 6.0 and 6.6 kb are required for the expression of ugp, whereas all the structural information is located on the 6.6 kb EcoRI fragment. A restriction map of the cloned DNA was established and the extent of ugp genes determined by Tn5 insertions. Using ugp-lacZ fusions, it could be shown that the ugp region consists of at least two different operons that are transcribed in the same direction (counterclockwise) on the E. coli chromosome.
利用一种针对甘油-3-磷酸（G3P）转运活性的新型阳性选择方法，从构建于λgt7的大肠杆菌EcoRI片段文库中分离出携带全部或部分ugp（大肠杆菌磷酸调节子依赖性G3P转运系统的基因）的λ噬菌体。通过将不同噬菌体携带的EcoRI片段亚克隆到多拷贝质粒pACYC184和pUR222中，结果表明ugp的表达需要两个分别为6.0 kb和6.6 kb的染色体片段，而所有的结构信息都位于6.6 kb的EcoRI片段上。构建了克隆DNA的限制性图谱，并通过Tn5插入确定了ugp基因的范围。利用ugp-lacZ融合技术，可以证明ugp区域至少由两个不同的操纵子组成，它们在大肠杆菌染色体上以相同方向（逆时针）转录。
