Vilja P, Krohn K, Tuohimaa P
J Immunol Methods. 1985 Jan 21;76(1):73-83. doi: 10.1016/0022-1759(85)90482-x.
We have developed an avidin-biotin assay for the detection of lactoferrin in human seminal plasma. We compared 5 modifications of this assay, and found the non-competitive avidin-biotin assay (NABA) to be the most sensitive. The detection limit of 3-step NABA was 0.2 or 0.1 ng lactoferrin/ml, depending whether avidin-biotin-peroxidase complex (ABC) or avidin-peroxidase was used. The intra-assay and inter-assay coefficients of variation for 3-step NABA were 6.2 and 8.5%, respectively. The lactoferrin levels of human seminal plasma samples measured by 3-step NABA and radioimmunoassay (RIA) showed good correlation (r = 0.96). The total performance time of 3-step NABA is flexible and the method can be modified for rapid (less than 1 h) or overnight assay according to need.
我们已开发出一种抗生物素蛋白-生物素检测法，用于检测人精浆中的乳铁蛋白。我们比较了该检测法的5种改进方法，发现非竞争性抗生物素蛋白-生物素检测法（NABA）最为灵敏。三步法NABA的检测限为0.2或0.1 ng乳铁蛋白/毫升，具体取决于使用的是抗生物素蛋白-生物素-过氧化物酶复合物（ABC）还是抗生物素蛋白-过氧化物酶。三步法NABA的批内和批间变异系数分别为6.2%和8.5%。通过三步法NABA和放射免疫分析法（RIA）测定的人精浆样本中的乳铁蛋白水平显示出良好的相关性（r = 0.96）。三步法NABA的总检测时间灵活，该方法可根据需要修改为快速检测（少于1小时）或过夜检测。
