Vilja P, Turunen H J, Leinikki P O
J Clin Microbiol. 1985 Oct;22(4):637-40. doi: 10.1128/jcm.22.4.637-640.1985.
A new capture enzyme immunoassay for the determination of immunoglobulin M (IgM) antibodies against hepatitis B core antigen (HBcAg) is described. Core antigen produced in Escherichia coli was labeled with biotin and subsequently detected by an avidin-biotin-peroxidase complex. The biotin-labeled core antigen was effective at concentrations as low as 20 ng/ml. Of 561 serum samples from different groups of patients that were tested, 465 samples were negative for other hepatitis B virus markers and also for anti-HBcAg IgM. Sera from the early stages of hepatitis B infection had high levels of anti-HBcAg IgM, and a clear correlation with the acuteness of the disease was observed in 45 follow-up sera from 23 patients with acute or recent hepatitis B. Sera from 21 patients with past hepatitis B were all negative for anti-HBcAg IgM. Twenty serum samples from chronic carriers of hepatitis B surface antigen showed slightly elevated antibody levels for anti-HBcAg IgM. Ten sera which were positive for anti-HBcAg IgG antibodies and had high levels of rheumatoid factor were negative for anti-HBcAg IgM.
本文描述了一种用于检测抗乙型肝炎核心抗原（HBcAg）免疫球蛋白M（IgM）抗体的新型捕获酶免疫测定法。在大肠杆菌中产生的核心抗原用生物素标记，随后通过抗生物素蛋白-生物素-过氧化物酶复合物进行检测。生物素标记的核心抗原在低至20 ng/ml的浓度下仍有效。在检测的来自不同患者组的561份血清样本中，465份样本的其他乙型肝炎病毒标志物及抗HBcAg IgM均为阴性。乙型肝炎感染早期的血清中抗HBcAg IgM水平较高，在23例急性或近期感染乙型肝炎患者的45份随访血清中观察到其与疾病的急性程度有明显相关性。21例既往感染过乙型肝炎患者的血清抗HBcAg IgM均为阴性。20份乙型肝炎表面抗原慢性携带者的血清样本显示抗HBcAg IgM抗体水平略有升高。10份抗HBcAg IgG抗体阳性且类风湿因子水平较高的血清抗HBcAg IgM为阴性。
