Charpin C, Lachard A, Pourreau-Schneider N, Jacquemier J, Lavaut M N, Andonian C, Martin P M, Toga M
Cancer. 1985 Jun 1;55(11):2612-7. doi: 10.1002/1097-0142(19850601)55:11<2612::aid-cncr2820551113>3.0.co;2-c.
A retrospective immunocytochemical study was performed on 67 human breast carcinomas to determine whether the epithelial cell-associated antigens, lactoferrin and nonspecific cross-reacting antigen (NCA), could be used as markers in the prognostic assessment of breast cancers. Fixed paraffin sections were tested with anti-lactoferrin and anti-NCA. Lactoferrin and NCA were found in 7.5% and 19% of the cases, respectively. Furthermore, the association between these two antigens in tumor cells was significant (P less than 0.05). Kappa-casein was observed in all antigen-positive cases. These antigens were observed more often in low-grade ductal carcinomas that had positive estrogen and progestin receptors, but no relationship could be established between lactoferrin or NCA and other prognostic indicators, such as histologic type and grade of the tumor, stromal elastosis, or steroid receptors. Although more antigen might have been detected in unfixed, frozen specimens, the results indicate that lactoferrin and NCA possess minimal value as epithelial cell markers and no prognostic value when detected on routinely fixed, paraffin-embedded samples.
对67例人类乳腺癌进行了一项回顾性免疫细胞化学研究，以确定上皮细胞相关抗原乳铁蛋白和非特异性交叉反应抗原（NCA）是否可作为乳腺癌预后评估的标志物。用抗乳铁蛋白和抗NCA对固定的石蜡切片进行检测。分别在7.5%和19%的病例中发现了乳铁蛋白和NCA。此外，肿瘤细胞中这两种抗原之间的关联具有显著性（P小于0.05）。在所有抗原阳性病例中均观察到κ-酪蛋白。这些抗原在雌激素和孕激素受体阳性的低级别导管癌中更常见，但乳铁蛋白或NCA与其他预后指标之间未发现关联，如肿瘤的组织学类型和分级、间质弹性组织变性或类固醇受体。尽管在未固定的冷冻标本中可能检测到更多抗原，但结果表明，乳铁蛋白和NCA作为上皮细胞标志物的价值极小，在常规固定、石蜡包埋样本上检测时无预后价值。
