Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, Brazil; Private Practice. Louveira, Brazil.
Private Practice.
J Stomatol Oral Maxillofac Surg. 2024 Oct;125(5S2):101923. doi: 10.1016/j.jormas.2024.101923. Epub 2024 May 28.
Chlorhexidine (CHX) is a prime choice to control the oral microbiota. However, it's a chemical agent leading to side effects such as teeth strains, taste disturbance, and desquamation of oral mucosa. Alternatively, the lactoferrin and oxygen-based Blue®M has been introduced as an alternative to the CHX, not disturbing tissue repair. Therefore, the study aimed to evaluate the effects of Blue®M and CHX on oral human fibroblasts (HGF-1) and keratinocytes (NOK-SI). Cell cultures using HGF-1 and NOK-SI evaluated cell proliferation, cell cycle, apoptosis and necrosis, and migration. In the dose-effect test, Blue®M reduced the HGF-1 sample in a 4-fold concentration than CHX (CHX: 173.07 ±10.27; Blue®M: 43.86 ±3.04). The proliferation test revealed an eightfold reduction of the sample for CHX, while for Blue®M, the proliferation rate was eighteen times lower. The apoptosis and necrosis rates increased by 25% (p<0.0001) for HGF-1 for both substances. In NOK-SI, the apoptosis rates increased by 10% (p=0.02) and 15% (p=0.001) for CHX and Blue®M, respectively. Furthermore, the fibroblast had a lower capacity for wound closure in the Scratch Assay (monolayer cell migration) for Blue®M. Despite the limitations of this in vitro study, the results of the lactoferrin and oxygen-based Blue®M demonstrated cytotoxicity in doses over the Minimum inhibitory concentration and Minimum bactericidal concentration for Oral fibroblasts (HGF- 1) and Keratinocytes (NOK-SI).
洗必泰（CHX）是控制口腔微生物群的首选。然而，它是一种化学药剂，会导致牙齿紧张、味觉障碍和口腔粘膜脱落等副作用。相反，乳铁蛋白和基于氧的 Blue®M 已被引入作为 CHX 的替代品，不会干扰组织修复。因此，本研究旨在评估 Blue®M 和 CHX 对口腔人成纤维细胞（HGF-1）和角质形成细胞（NOK-SI）的影响。使用 HGF-1 和 NOK-SI 的细胞培养物评估细胞增殖、细胞周期、细胞凋亡和坏死以及迁移。在剂量效应试验中，Blue®M 将 HGF-1 样本的浓度降低到 CHX 的 4 倍（CHX：173.07±10.27；Blue®M：43.86±3.04）。增殖试验显示 CHX 样本的增殖减少了 8 倍，而 Blue®M 的增殖率则降低了 18 倍。两种物质均使 HGF-1 的细胞凋亡和坏死率增加了 25%（p<0.0001）。在 NOK-SI 中，CHX 和 Blue®M 的细胞凋亡率分别增加了 10%（p=0.02）和 15%（p=0.001）。此外，Blue®M 处理的成纤维细胞在划痕试验（单层细胞迁移）中对伤口闭合的能力较低。尽管这项体外研究存在局限性，但乳铁蛋白和基于氧的 Blue®M 的结果表明，在口腔成纤维细胞（HGF-1）和角质形成细胞（NOK-SI）的最低抑菌浓度和最低杀菌浓度以上的剂量下，具有细胞毒性。
