Virina A M, Feĭgin A M, Belousova I I, Tereshin I M
Mikrobiologiia. 1979 Sep-Oct;48(5):838-44.
The lipid and protein composition of Candida albicans, strain R3, with a low resistance to polyene antibiotics does not differ much from that of the parent, susceptible culture. Nevertheless, the rate of potassium release from the cells and the incorporation rate of 14C-amino acids in the cells of C. albicans, strain R3, under the action of amphotericin B are lower as compared to the susceptible culture; therefore, the resistance of C. albicans, strain R3, must be due to a decrease in the degress of its disordered selective permeability. Sterols isolated from the cells in both strains were found to be capable of binding to polyenes at a high rate in solution. However, sterols from the strain R3 with a low resistance to polyenes, when incorporated into a synthetic lipid membrane, increase its permeability slower than sterols from the susceptible strain, which suggests that sterols from the low-resistant strain R3 are possibility involved in the phenotypic manifestation of resistance.
对多烯抗生素耐药性较低的白色念珠菌R3菌株的脂质和蛋白质组成与亲代敏感培养物的脂质和蛋白质组成差异不大。然而，在两性霉素B的作用下，白色念珠菌R3菌株细胞的钾释放速率和14C-氨基酸掺入细胞的速率低于敏感培养物；因此，白色念珠菌R3菌株的耐药性必定是由于其无序选择性通透性的程度降低所致。在两种菌株的细胞中分离出的甾醇在溶液中都能够与多烯以高比率结合。然而，对多烯耐药性较低的R3菌株的甾醇在掺入合成脂质膜时，其增加膜通透性的速度比敏感菌株的甾醇慢，这表明低耐药性R3菌株的甾醇可能参与了耐药性的表型表现。
