[多烯抗生素对白色念珠菌游离核糖体和膜结合核糖体蛋白质合成的影响]

[Effect of polyene antibiotics on protein synthesis by free and membrane-bound ribosomes of Candida albicans].

作者信息

Lishnevskaia E B, Belousova I I, Rodygina T N, Tereshin I M

出版信息

Biokhimiia. 1976;41(7):1169-75.

Abstract

Polyene antibiotics (levorin, nistatin, amphotericin B) inhibit protein synthesis at concentrations decreasing 14C-amino acid incorporation into Candida albicans protoplasts by 30--60%, the depression of membrane permeability beginning earlier than protein synthesis inhibition. Fractionation of protoplast lysates revealed that protein synthesis by free ribosomes was inhibited by antibiotics stronger than in case of membrane-bound ribosomes. It is supposed that different response of two ribosome classes for polyenes-induced damages is due to different sensitivity of free and membrane-bound ribosomes to the decrease of intracellular K+ concentration.

摘要

多烯类抗生素（制霉菌素、制念菌素、两性霉素B）在浓度降低时抑制蛋白质合成，使14C - 氨基酸掺入白色念珠菌原生质体的量减少30% - 60%，膜通透性的降低比蛋白质合成抑制开始得更早。原生质体裂解物的分级分离显示，游离核糖体的蛋白质合成比膜结合核糖体更易受到抗生素的抑制。据推测，两类核糖体对多烯诱导损伤的不同反应是由于游离核糖体和膜结合核糖体对细胞内钾离子浓度降低的敏感性不同。