Nestmann E R, Lee E G
Mutat Res. 1985 Jan-Feb;155(1-2):53-60. doi: 10.1016/0165-1218(85)90025-4.
20 compounds identified in pulp mill effluents were screened for genetic activity in growing cells using Saccharomyces cerevisiae strains D7 and XV185-14C without and with S9. Nine compounds were positive in one or the other yeast strain (7 in D7; 2 in XV185-14C). One additional compound showed weak effects and two others showed elevated frequencies/survivor without absolute increases of mutants. The presence of S9 enabled detection of one positive and two weak effects, it enhanced the genetic activity of one compound in each strain, and it reduced the mutagenic effects of 4 others in strain D7. 7 of the 20 chemicals tested have been shown previously to be mutagenic in the Salmonella/mammalian-microsome assay. Of the 7 bacterial mutagens, 6 were positive and 1 had a weak effect in yeast.
利用酿酒酵母菌株D7和XV185 - 14C，在有无S9的情况下，对纸浆厂废水中鉴定出的20种化合物进行生长细胞遗传活性筛选。9种化合物在一种或另一种酵母菌株中呈阳性（7种在D7中；2种在XV185 - 14C中）。另外一种化合物显示出微弱效应，还有两种化合物显示突变频率/存活率升高，但突变体没有绝对增加。S9的存在使得能够检测到一种阳性和两种微弱效应，它增强了每种菌株中一种化合物的遗传活性，并且降低了菌株D7中其他4种化合物的诱变效应。之前已证明，所测试的20种化学物质中有7种在沙门氏菌/哺乳动物微粒体试验中具有致突变性。在这7种细菌诱变剂中，6种在酵母中呈阳性，1种有微弱效应。
