China Three Gorges University, Yichang, China.
The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China.
PeerJ. 2024 May 27;12:e17410. doi: 10.7717/peerj.17410. eCollection 2024.
The basic helix-loop-helix (bHLH) gene family is integral to various aspects of plant development and the orchestration of stress response. This study focuses on the bHLH genes within , a poplar species noted for its significant tolerance to cadmium (Cd) stress. Through our comprehensive genomic analysis, we have identified and characterized 170 genes within the genome. These genes have been systematically classified into 22 distant subfamilies based on their evolutionary relationships. A notable conservation in gene structure and motif compositions were conserved across these subfamilies. Further analysis of the promoter regions of these genes revealed an abundance of essential cis-acting element, which are associated with plant hormonal regulation, development processes, and stress response pathway. Utilizing quantitative PCR (qPCR), we have documented the differential regulation of PcbHLHs in response to elevated Cd concentrations, with distinct expression patterns observed across various tissues. This study is poised to unravel the molecular mechanism underpinning Cd tolerance in , offering valuable insights for the development of new cultivars with enhanced Cd accumulation capacity and tolerance. Such advancements are crucial for implementing effective phytoremediation strategies to mitigate soil pollution caused by Cd.
基本螺旋-环-螺旋 (bHLH) 基因家族是植物发育和应激反应协调的各个方面的重要组成部分。本研究集中在杨树物种 中的 bHLH 基因，该物种以对镉 (Cd) 胁迫具有显著的耐受性而闻名。通过全面的基因组分析，我们在 基因组中鉴定和描述了 170 个基因。这些基因根据其进化关系被系统地分类为 22 个远缘亚家族。这些亚家族之间保守了基因结构和基序组成的显著保守性。对这些基因启动子区域的进一步分析揭示了丰富的必需顺式作用元件，这些元件与植物激素调节、发育过程和应激反应途径有关。利用定量 PCR(qPCR)，我们记录了 PcbHLHs 在应对升高的 Cd 浓度时的差异调节，在不同组织中观察到不同的表达模式。这项研究有望揭示 中 Cd 耐受性的分子机制，为开发具有增强 Cd 积累能力和耐受性的新品种提供有价值的见解。这些进展对于实施有效的植物修复策略以减轻 Cd 引起的土壤污染至关重要。
